Anti-Glucokinase抗体(ab70857)

概述

  • 产品名称Anti-Glucokinase抗体
    参阅全部 Glucokinase 一抗
  • 描述
    兔多克隆抗体to Glucokinase
  • 经测试应用适用于: ELISA, WB, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Synthetic peptide corresponding to Human Glucokinase (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH).
    Database link: P35557

  • 阳性对照
    • mouse lung tissue lysate, human breast carcinoma tissue

性能

应用

Our Abpromise guarantee covers the use of ab70857 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/1000.
WB 1/100 - 1/500. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa).
IHC-P 1/50 - 1/100.

靶标

  • 功能Catalyzes the initial step in utilization of glucose by the beta-cell and liver at physiological glucose concentration. Glucokinase has a high Km for glucose, and so it is effective only when glucose is abundant. The role of GCK is to provide G6P for the synthesis of glycogen. Pancreatic glucokinase plays an important role in modulating insulin secretion. Hepatic glucokinase helps to facilitate the uptake and conversion of glucose by acting as an insulin-sensitive determinant of hepatic glucose usage.
  • 组织特异性Isoform 1 is expressed in pancreas. Isoform 2 and isoform 3 is expressed in liver.
  • 疾病相关Defects in GCK are the cause of maturity-onset diabetes of the young type 2 (MODY2) [MIM:125851]; also shortened MODY-2. MODY is a form of diabetes that is characterized by an autosomal dominant mode of inheritance, onset in childhood or early adulthood (usually before 25 years of age), a primary defect in insulin secretion and frequent insulin-independence at the beginning of the disease.
    Defects in GCK are the cause of familial hyperinsulinemic hypoglycemia type 3 (HHF3) [MIM:602485]; also known as persistent hyperinsulinemic hypoglycemia of infancy (PHHI) or congenital hyperinsulinism. HHF is the most common cause of persistent hypoglycemia in infancy. Unless early and aggressive intervention is undertaken, brain damage from recurrent episodes of hypoglycemia may occur.
  • 序列相似性Belongs to the hexokinase family.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ATP:D-hexose 6-phosphotransferase antibody
    • FGQTL3 antibody
    • GCK antibody
    • GK antibody
    • GLK antibody
    • Glucokinase antibody
    • Hexokinase D pancreatic isozyme antibody
    • Hexokinase type IV antibody
    • Hexokinase-4 antibody
    • Hexokinase-D antibody
    • HHF3 antibody
    • HK IV antibody
    • HK4 antibody
    • HKIV antibody
    • HXK4_HUMAN antibody
    • HXKP antibody
    • LGLK antibody
    • MODY2 antibody
    see all

Anti-Glucokinase antibody 图像

  • Anti-Glucokinase antibody (ab70857) at 1/100 dilution + mouse lung tissue lysate at 12.5 µg

    Predicted band size : 52 kDa
    Observed band size : 52 kDa
    Additional bands at : 40 kDa,60 kDa,70 kDa. We are unsure as to the identity of these extra bands.
  • Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with 1/50 ab70857. A peroxidase-conjugated secondary antibody was then used, followed by AEC staining.

Anti-Glucokinase antibody (ab70857)参考文献

ab70857 has not yet been referenced specifically in any publications.

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