Anti-Glucocorticoid Receptor (phospho S226)抗体(ab93104)

概述

  • 产品名称Anti-Glucocorticoid Receptor (phospho S226)抗体
    参阅全部 Glucocorticoid Receptor 一抗
  • 描述
    兔多克隆抗体to Glucocorticoid Receptor (phospho S226)
  • 经测试应用适用于: IHC-P, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Sheep, Rabbit, Horse, Cow, Dog, Pig, Monkey, Orangutan
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 200 - 300 of Human Glucocorticoid Receptor, phosphorylated at S226 and phosphorylated at S226.

    (Peptide available as ab109640.)

  • 阳性对照
    • This antibody gave a positive signal in A549 whole cell lysate. IHC-P: Human skeletal muscle FFPE tissue sections.

性能

应用

Our Abpromise guarantee covers the use of ab93104 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 95 kDa (predicted molecular weight: 85 kDa).

The glucocorticoid receptor contains a number of potential phosphorylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.

靶标

  • 功能Receptor for glucocorticoids (GC). Has a dual mode of action: as a transcription factor that binds to glucocorticoid response elements (GRE) and as a modulator of other transcription factors. Affects inflammatory responses, cellular proliferation and differentiation in target tissues. Could act as a coactivator for STAT5-dependent transcription upon growth hormone (GH) stimulation and could reveal an essential role of hepatic GR in the control of body growth. Involved in chromatin remodeling. Plays a significant role in transactivation. Involved in nuclear translocation.
  • 组织特异性Widely expressed. In the heart, detected in left and right atria, left and right ventricles, aorta, apex, intraventricular septum, and atrioventricular node as well as whole adult and fetal heart.
  • 疾病相关Defects in NR3C1 are a cause of glucocorticoid resistance (GCRES) [MIM:138040]; also known as cortisol resistance. It is a hypertensive, hyperandrogenic disorder characterized by increased serum cortisol concentrations. Inheritance is autosomal dominant.
  • 序列相似性Belongs to the nuclear hormone receptor family. NR3 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • 结构域Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
  • 翻译后修饰Increased proteasome-mediated degradation in response to glucocorticoids.
    Phosphorylated in the absence of hormone; becomes hyperphosphorylated in the presence of glucocorticoid. The Ser-203-phosphorylated form is mainly cytoplasmic, and the Ser-211-phosphorylated form is nuclear. Transcriptional activity correlates with the amount of phosphorylation at Ser-211.
    Sumoylated; this reduces transcription transactivation.
    Ubiquitinated; restricts glucocorticoid-mediated transcriptional signaling.
  • 细胞定位Cytoplasm. Nucleus. Cytoplasmic in the absence of ligand, nuclear after ligand-binding and Nucleus. Localized largely in the nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • GCCR antibody
    • GCR antibody
    • GCR_HUMAN antibody
    • GCRST antibody
    • glucocorticoid nuclear receptor variant 1 antibody
    • Glucocorticoid receptor antibody
    • GR antibody
    • GRL antibody
    • Grl1 antibody
    • nr3c1 antibody
    • Nuclear receptor subfamily 3 group C member 1 antibody
    • nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor) antibody
    see all

Anti-Glucocorticoid Receptor (phospho S226) antibody 图像

  • IHC image of Glucocorticoid Receptor (phospho S226) staining in human skeletal muscle formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab93104, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
  • All lanes : Anti-Glucocorticoid Receptor (phospho S226) antibody (ab93104) at 1 µg/ml

    Lane 1 : A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate
    Lane 2 : A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate with with immunizing peptide at 1 µg/ml
    Lane 3 : A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate with with non modified peptide at 1 µg/ml

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 85 kDa
    Observed band size : 95 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 16 kDa,35 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 8 minutes
  • All lanes : Anti-Glucocorticoid Receptor (phospho S226) antibody (ab93104) at 1 µg/ml

    Lane 1 : A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate
    Lane 2 : A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate with immunizing peptide at 1 µg/ml
    Lane 3 : A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate with non modified peptide at 1 µg/ml

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 85 kDa
    Observed band size : 85 kDa


    Exposure time : 20 minutes

Anti-Glucocorticoid Receptor (phospho S226) antibody (ab93104)参考文献

ab93104 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your reply.

Your protocol seems fine. The recommended dilution for the primary antibody would be 1/200. Thus, if you haven't tried that I would suggest doing it.
Also, where do you see the faint bands, at which MW?
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