Anti-Glucocorticoid Receptor (phospho S211)抗体(ab55189)


  • 产品名称Anti-Glucocorticoid Receptor (phospho S211)抗体
    参阅全部 Glucocorticoid Receptor 一抗
  • 描述
    兔多克隆抗体to Glucocorticoid Receptor (phospho S211)
  • 特异性Detects endogenous levels of the Glucocorticoid Receptor only when phosphorylated at serine 211 (Mouse Ser220, Rat Ser232)
  • 经测试应用适用于: IHC-P, WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Synthetic phosphopeptide derived from the human Glucocorticoid Receptor around the phosphorylation site of serine 211 (NESPPW).

  • 阳性对照
    • Human breast carcinoma tissue, extracts from HeLa cells treated with PMA



Our Abpromise guarantee covers the use of ab55189 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB 1/500 - 1/1000. Detects a band of approximately 86 kDa (predicted molecular weight: 86 kDa).
ELISA 1/10000.


  • 功能Receptor for glucocorticoids (GC). Has a dual mode of action: as a transcription factor that binds to glucocorticoid response elements (GRE) and as a modulator of other transcription factors. Affects inflammatory responses, cellular proliferation and differentiation in target tissues. Could act as a coactivator for STAT5-dependent transcription upon growth hormone (GH) stimulation and could reveal an essential role of hepatic GR in the control of body growth. Involved in chromatin remodeling. Plays a significant role in transactivation. Involved in nuclear translocation.
  • 组织特异性Widely expressed. In the heart, detected in left and right atria, left and right ventricles, aorta, apex, intraventricular septum, and atrioventricular node as well as whole adult and fetal heart.
  • 疾病相关Defects in NR3C1 are a cause of glucocorticoid resistance (GCRES) [MIM:138040]; also known as cortisol resistance. It is a hypertensive, hyperandrogenic disorder characterized by increased serum cortisol concentrations. Inheritance is autosomal dominant.
  • 序列相似性Belongs to the nuclear hormone receptor family. NR3 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • 结构域Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
  • 翻译后修饰Increased proteasome-mediated degradation in response to glucocorticoids.
    Phosphorylated in the absence of hormone; becomes hyperphosphorylated in the presence of glucocorticoid. The Ser-203-phosphorylated form is mainly cytoplasmic, and the Ser-211-phosphorylated form is nuclear. Transcriptional activity correlates with the amount of phosphorylation at Ser-211.
    Sumoylated; this reduces transcription transactivation.
    Ubiquitinated; restricts glucocorticoid-mediated transcriptional signaling.
  • 细胞定位Cytoplasm. Nucleus. Cytoplasmic in the absence of ligand, nuclear after ligand-binding and Nucleus. Localized largely in the nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • GCCR antibody
    • GCR antibody
    • GCR_HUMAN antibody
    • GCRST antibody
    • glucocorticoid nuclear receptor variant 1 antibody
    • Glucocorticoid receptor antibody
    • GR antibody
    • GRL antibody
    • Grl1 antibody
    • nr3c1 antibody
    • Nuclear receptor subfamily 3 group C member 1 antibody
    • nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor) antibody
    see all

Anti-Glucocorticoid Receptor (phospho S211) antibody 图像

  • All lanes : Anti-Glucocorticoid Receptor (phospho S211) antibody (ab55189) at 1/500 dilution

    Lane 1 : extracts from HeLa cells treated with PMA (125ng/ml, 30min), with the immunising phosphpeptide.
    Lane 2 : extracts from HeLa cells treated with PMA (125ng/ml, 30min).

    Predicted band size : 86 kDa
    Observed band size : 86 kDa
  • Ab55189 at 1/50 dilution staining human breast carcinoma without (left) and with (right) immunizing phospho-peptide; paraffin embedded.

Anti-Glucocorticoid Receptor (phospho S211) antibody (ab55189)参考文献

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I have been informed that rat tissue was not tested by the laboratory, but that it is predicted to cross react due to the high homology of the immunogen and the rat protein. I have therefore removed this species from the datasheet and arranged a credi...

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