Anti-Glucocorticoid Receptor beta抗体(ab3581)




Our Abpromise guarantee covers the use of ab3581 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 5 µg/ml.

Fixation: 4% paraformaldehyde for 15 minutes at room temperature. Permeabilisation: PBS containing 0.01% saponin for 15 mins Blocking: 1% BSA in PBS Primary: overnight at 4ºC

EMSA Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. PubMed: 20438742Sample Preparation: endogenous peroxidase blocked with 0.3% hydrogen peroxide. Antigen retrieval: Heat induced antigen retrieval performed in 0.01M Tris-EDTA pH 9.0. Primary antibody: overnight at 4°C
Flow Cyt Use at an assay dependent concentration. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
ICC 1/1500.
IP Use at an assay dependent concentration. Sample preparation: RIPA buffer containing protease inhibitors was added to cell lysate. Primary antibody: 1:10 dilution for 2 h on ice Prewashed magnetic beads added to lysate mixture and incubated overnight at +4ºC. After washing, sample reducing buffer was added and boiled (5 min, 95ºC)
WB 1/500. Detects a band of approximately 90 kDa (predicted molecular weight: 83 kDa).Can be blocked with Human Glucocorticoid Receptor beta peptide (ab39765). Sample preparation: Lysates boiled in reducing buffer for 5 min. 20µg of protein loaded Blocking agent: 5% non-fat milk in Tris buffered saline (TBS) Primary antibody: overnight at 4°C


  • 功能Receptor for glucocorticoids (GC). Has a dual mode of action: as a transcription factor that binds to glucocorticoid response elements (GRE) and as a modulator of other transcription factors. Affects inflammatory responses, cellular proliferation and differentiation in target tissues. Could act as a coactivator for STAT5-dependent transcription upon growth hormone (GH) stimulation and could reveal an essential role of hepatic GR in the control of body growth. Involved in chromatin remodeling. Plays a significant role in transactivation. Involved in nuclear translocation.
  • 组织特异性Widely expressed. In the heart, detected in left and right atria, left and right ventricles, aorta, apex, intraventricular septum, and atrioventricular node as well as whole adult and fetal heart.
  • 疾病相关Defects in NR3C1 are a cause of glucocorticoid resistance (GCRES) [MIM:138040]; also known as cortisol resistance. It is a hypertensive, hyperandrogenic disorder characterized by increased serum cortisol concentrations. Inheritance is autosomal dominant.
  • 序列相似性Belongs to the nuclear hormone receptor family. NR3 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • 结构域Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
  • 翻译后修饰Increased proteasome-mediated degradation in response to glucocorticoids.
    Phosphorylated in the absence of hormone; becomes hyperphosphorylated in the presence of glucocorticoid. The Ser-203-phosphorylated form is mainly cytoplasmic, and the Ser-211-phosphorylated form is nuclear. Transcriptional activity correlates with the amount of phosphorylation at Ser-211.
    Sumoylated; this reduces transcription transactivation.
    Ubiquitinated; restricts glucocorticoid-mediated transcriptional signaling.
  • 细胞定位Cytoplasm. Nucleus. Cytoplasmic in the absence of ligand, nuclear after ligand-binding and Nucleus. Localized largely in the nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • GCCR antibody
    • GCR antibody
    • GCR_HUMAN antibody
    • glucocorticoid nuclear receptor variant 1 antibody
    • Glucocorticoid receptor antibody
    • Glucocorticoid receptor beta isoform antibody
    • GR antibody
    • GRL antibody
    • nr3c1 antibody
    • Nuclear receptor subfamily 3 group C member 1 antibody
    see all

Anti-Glucocorticoid Receptor beta antibody 图像

  • ICC/IF image of ab3581 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab3581, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Anti-Glucocorticoid Receptor beta antibody (ab3581) at 1/500 dilution + COS-74 whole cell extract transfected with Glucocorticoid Receptor beta

    Predicted band size : 83 kDa

Anti-Glucocorticoid Receptor beta antibody (ab3581)参考文献

This product has been referenced in:
  • Butler CA  et al. Glucocorticoid receptor ß and histone deacetylase 1 and 2 expression in the airways of severe asthma. Thorax 67:392-8 (2012). ICC/IF ; Human . Read more (PubMed: 22156779) »
  • Melarangi T  et al. Glucocorticoid resistance in chronic lymphocytic leukaemia is associated with a failure of upregulated Bim/Bcl-2 complexes to activate Bax and Bak. Cell Death Dis 3:e372 (2012). WB ; Human . Read more (PubMed: 22898870) »

See all 4 Publications for this product

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