The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Detects a band of approximately 86 kDa (predicted molecular weight: 86 kDa).
Use at an assay dependent concentration.
Use a concentration of 5 µg/ml.
Receptor for glucocorticoids (GC). Has a dual mode of action: as a transcription factor that binds to glucocorticoid response elements (GRE) and as a modulator of other transcription factors. Affects inflammatory responses, cellular proliferation and differentiation in target tissues. Could act as a coactivator for STAT5-dependent transcription upon growth hormone (GH) stimulation and could reveal an essential role of hepatic GR in the control of body growth. Involved in chromatin remodeling. Plays a significant role in transactivation. Involved in nuclear translocation.
Widely expressed. In the heart, detected in left and right atria, left and right ventricles, aorta, apex, intraventricular septum, and atrioventricular node as well as whole adult and fetal heart.
Defects in NR3C1 are a cause of glucocorticoid resistance (GCRES) [MIM:138040]; also known as cortisol resistance. It is a hypertensive, hyperandrogenic disorder characterized by increased serum cortisol concentrations. Inheritance is autosomal dominant.
Belongs to the nuclear hormone receptor family. NR3 subfamily. Contains 1 nuclear receptor DNA-binding domain.
Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
Increased proteasome-mediated degradation in response to glucocorticoids. Phosphorylated in the absence of hormone; becomes hyperphosphorylated in the presence of glucocorticoid. The Ser-203-phosphorylated form is mainly cytoplasmic, and the Ser-211-phosphorylated form is nuclear. Transcriptional activity correlates with the amount of phosphorylation at Ser-211. Sumoylated; this reduces transcription transactivation. Ubiquitinated; restricts glucocorticoid-mediated transcriptional signaling.
Cytoplasm. Nucleus. Cytoplasmic in the absence of ligand, nuclear after ligand-binding and Nucleus. Localized largely in the nucleus.
Nuclear receptor subfamily 3 group C member 1 antibody
nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor) antibody
Western blot - Glucocorticoid Receptor antibody (ab55400)
All lanes : Anti-Glucocorticoid Receptor antibody (ab55400) at 1/500 dilution
Lane 1 : Jurkat cell extract, treated
with EGF (200ng/ml, 15mins), without immunizing peptide Lane 2 : Jurkat cell extract, treated
with EGF (200ng/ml, 15mins), with immunizing peptide
Predicted band size : 86 kDa Observed band size : 86 kDa
Immunoprecipitation - Glucocorticoid Receptor antibody (ab55400)This image is courtesy of an Anonymous Abreview.
ab55400 immunoprecipitating Glucocorticoid Receptor from Hepa1-6 (murine hepatoma) cells. 1 mg of cell lysate was incubated with the primary antibody and matrix (Protein A) at a concentration of 20 µl/mg lysate for 18 hours at 4°C in cell lysis buffer. Immunoprecipitate was then Western blotted with ab55400 at 1/500.
ICC/IF image of ab55400 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab55400, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.