Anti-Glucagon Receptor抗体(ab75240)


  • 产品名称Anti-Glucagon Receptor抗体
    参阅全部 Glucagon Receptor 一抗
  • 描述
    兔多克隆抗体to Glucagon Receptor
  • 经测试应用适用于: WB, ELISA, ICC/IF, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Synthesized peptide derived from internal of human Glucagon Receptor

  • 阳性对照
    • extracts from COLO205 cells; HeLa cells




Our Abpromise guarantee covers the use of ab75240 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Predicted molecular weight: 54 kDa.Can be blocked with Glucagon Receptor peptide (ab140072).
ELISA 1/5000.
ICC/IF 1/500 - 1/1000.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.


Anti-Glucagon Receptor antibody 图像

  • All lanes : Anti-Glucagon Receptor antibody (ab75240) at 1/500 dilution

    Lane 1 : extracts from COLO205 cells
    Lane 2 : extracts from COLO205 cells with immunizing peptide at 5 µg

    Lysates/proteins at 15 µg per lane.

    Predicted band size : 54 kDa
    Observed band size : 54 kDa
  • Left panel: immunofluorescence analysis of HeLa cells with 1/500 ab75240. Right panel: same, after pre-incubation with the immunizing peptide.
  • IHC image of ab75240 staining in human normal kidney formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab75240, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-Glucagon Receptor antibody (ab75240)参考文献

This product has been referenced in:
  • Komurov K  et al. The glucose-deprivation network counteracts lapatinib-induced toxicity in resistant ErbB2-positive breast cancer cells. Mol Syst Biol 8:596 (2012). WB ; Human . Read more (PubMed: 22864381) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Liver)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citric acid
Permeabilization Yes - H2O2
Specification Liver
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Fixative Paraformaldehyde

Abcam user community

Verified customer

提交于 Sep 27 2016

Unfortunately we do not offer gold conjugation services. We do, however, provide gold conjugated secondaries, like Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (5nm Gold), pre-adsorbed, ab27235

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Thank you for contacting us. We have added the blocking peptide for ab75240 to our catalog as ab140072. I hope this helps, please let me know if you need any additional information or assistance.

Thank you for your inquiry.

Unfortunately, we are not able to release the immunogen sequence for this particular antibody as it is proprietary information.

We aim to provide as much information as possible to our customers, so I a...

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