Anti-Glucagon Receptor抗体(ab109279)
Key features and details
- Rabbit polyclonal to Glucagon Receptor
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-Glucagon Receptor抗体
参阅全部 Glucagon Receptor 一抗 -
描述
兔多克隆抗体to Glucagon Receptor -
宿主
Rabbit -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human
预测可用于: Macaque monkey, Gorilla -
免疫原
Synthetic peptide corresponding to Human Glucagon Receptor aa 300-400 conjugated to keyhole limpet haemocyanin.
(Peptide available asab129210) -
阳性对照
- This antibody gave a positive signal in the following whole cell lysates: Caco2; HepG2; SW480.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab109279于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/250. Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa).
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说明 |
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WB
1/250. Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa). |
靶标
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功能
This is a receptor for glucagon which plays a central role in regulating the level of blood glucose by controlling the rate of hepatic glucose production and insulin secretion. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase and also a phosphatidylinositol-calcium second messenger system. -
序列相似性
Belongs to the G-protein coupled receptor 2 family. -
细胞定位
Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 2642 Human
- Omim: 138033 Human
- SwissProt: P47871 Human
- Unigene: 208 Human
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别名
- Gcgr antibody
- GGR antibody
- GL R antibody
see all
图片
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All lanes : Anti-Glucagon Receptor antibody (ab109279) at 1/250 dilution (Milk Blocking - 3%)
Lane 1 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate
Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 3 : SW480 (Human colon adenocarcinoma cell line) Whole Cell Lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 54 kDa
Observed band size: 54 kDa
Additional bands at: 25 kDa, 51 kDa (possible cleavage fragment). We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes
The band observed at 51 kDa could potentially be a cleaved form of Glucagon Receptor due to the presence of a 25 amino acid signal peptide.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab109279 尚未被引用在任何文献中。