Anti-Glucagon抗体[K79bB10] (ab10988)
Key features and details
- Mouse monoclonal [K79bB10] to Glucagon
- Suitable for: IHC-P
- Reacts with: Rat
- Isotype: IgG1
概述
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产品名称
Anti-Glucagon抗体[K79bB10]
参阅全部 Glucagon 一抗 -
描述
小鼠单克隆抗体[K79bB10] to Glucagon -
宿主
Mouse -
经测试应用
适用于: IHC-Pmore details -
种属反应性
与反应: Rat
预测可用于: Rabbit, Guinea pig, Cat, Dog, Human, Pig, Common marmoset, Syrian hamster -
免疫原
Full length protein corresponding to Pig Glucagon. Polymerized porcine glucagon.
Database link: P01275 -
阳性对照
- Pancreatic glucagon for RIA. IHC-P: Mouse and Rat pancreas tissues.
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常规说明
Production of this antibody has been changed on 8th July 2016. The following lots are from ascites and are still in stock as of 8th July 2016: GR260160 and GR268340. Lot numbers higher than GR268340 will be purified from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly.
If slight turbidity occurs upon prolonged storage, clarify by centrifugation before use.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: PBS -
Concentration information loading...
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纯度
Proprietary Purification -
纯化说明
Purified from Tissue culture supernatant. -
克隆
单克隆 -
克隆编号
K79bB10 -
同种型
IgG1 -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab10988于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-P | (10) |
Use at an assay dependent concentration.
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说明 |
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IHC-P
Use at an assay dependent concentration. |
靶标
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相关性
Glucagon is a hormone that is secreted by alpha cells in the pancreas. Glucagon antagonizes insulin by converting glycogen to glucose in the liver and increasing blood sugar levels. Glucagon-like peptide 1 (GLP1), Glucagon-like peptide 2 (GLP2), VIP (vasoactive intestinal peptide) and PACAP (pituitary adenylate cyclase activating polypeptide) are in the glucagons hormone family. GLP1 is a transmitter in the central nervous system that regulates feeding and drinking behavior. GLP2 stimulates intestinal epithelial growth. -
细胞定位
Secreted -
数据库链接
- Entrez Gene: 403571 Dog
- Entrez Gene: 2641 Human
- Entrez Gene: 397595 Pig
- Entrez Gene: 24952 Rat
- Omim: 138030 Human
- SwissProt: P01275 Human
- SwissProt: P01274 Pig
- SwissProt: P68274 Rabbit
see all -
别名
- GCG antibody
图片
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Formali-fixed, paraffin-embedded rat pancreas tissue stained for Glucagon using ab10988 at 10 µg/ml in immunohistochemical analysis.
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ab10988 staining Glucagon in rat pancreatic tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
Tissue was fixed in paraformaldehyde, blocked with 2% serum for 1 hour at 25°C, then incubated with ab10988 at a 1/200 dilution for 1 hour at 25°C. The secondary used was a donkey anti-mouse IgG (H+L) conjugated to Texas Red, used at a 1/250 dilution. Nuclei counterstained with DAPI, green is staining for insulin.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (170)
ab10988 被引用在 170 文献中.
- Syed F et al. A discovery-based proteomics approach identifies protein disulphide isomerase (PDIA1) as a biomarker of β cell stress in type 1 diabetes. EBioMedicine 87:104379 (2023). PubMed: 36463755
- Shyr ZA et al. SGLT2 inhibitors therapy protects glucotoxicity-induced β-cell failure in a mouse model of human KATP-induced diabetes through mitigation of oxidative and ER stress. PLoS One 17:e0258054 (2022). PubMed: 35180212
- Cujba AM et al. An HNF1α truncation associated with maturity-onset diabetes of the young impairs pancreatic progenitor differentiation by antagonizing HNF1β function. Cell Rep 38:110425 (2022). PubMed: 35235779
- Katz LS et al. Maladaptive positive feedback production of ChREBPβ underlies glucotoxic β-cell failure. Nat Commun 13:4423 (2022). PubMed: 35908073
- Pedraza-Arevalo S et al. Differentiation of beta-like cells from human induced pluripotent stem cell-derived pancreatic progenitor organoids. STAR Protoc 3:101656 (2022). PubMed: 36092820