Anti-Glucagon抗体[K79bB10] (ab10988)

概述

  • 产品名称Anti-Glucagon抗体[K79bB10]
    参阅全部 Glucagon 一抗
  • 描述
    小鼠单克隆抗体[K79bB10] to Glucagon
  • 经测试应用适用于: IHC-P, ICC/IF, IHC-Fr, RIA, Dot blotmore details
  • 种属反应性
    与反应: Mouse, Rat, Dog, Human, Pig, Marmoset (common)
    预测可用于: Rabbit, Guinea pig, Cat, Syrian Hamster
  • 免疫原

    Polymerized porcine glucagon.

  • 阳性对照
    • Pancreatic glucagon for RIA.
  • 常规说明

    Production of this antibody has been changed on 8th July 2016. The following lots are from ascites and are still in stock as of 8th July 2016: GR260160 and GR268340.  Lot numbers higher than GR268340 will be purified from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly.

    If slight turbidity occurs upon prolonged storage, clarify by centrifugation before use.

性能

应用

Our Abpromise guarantee covers the use of ab10988 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration. PubMed: 21821034
RIA Use at an assay dependent concentration.
Dot blot Use at an assay dependent concentration.

靶标

Anti-Glucagon antibody [K79bB10] 图像

  • Immunohistochemical analysis of Mouse pancreas tissue labeling Glucagon with ab10988 at 1/1000 dilution.

  • Immunohistochemical analysis of Mouse pancreas tissue sections labeling Glucagon with ab10988 at 1/1000 dilution

  • ab10988 staining Glucagon in rat pancreatic tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
    Tissue was fixed in paraformaldehyde, blocked with 2% serum for 1 hour at 25°C, then incubated with ab10988 at a 1/200 dilution for 1 hour at 25°C. The secondary used was a donkey anti-mouse IgG (H+L) conjugated to Texas Red, used at a 1/250 dilution. Nuclei counterstained with DAPI, green is staining for insulin.

    See Abreview

  • ab10988 at 1/200 staining mouse pancreas tissue sections by IHC-P. The tissue sections were paraformaldehyde fixed and blocked with serum prior to incubation with the antibody for 16 hours at 4°C. A Texas Red conjugated donkey anti-mouse antibody was used as the secondary.

    See Abreview

  • ab10988 staining human islets (beta cells) by ICC/IF.  Cells were PFA fixed and blocked in 2% serum for 1 hour at 25°C prior to incubating with ab10988 for 1 hour at 25°C.  A Cy3® conjugated goat anti-mouse antibody diluted 1/500 was used as the secondary.

    See Abreview

Anti-Glucagon antibody [K79bB10] (ab10988)参考文献

This product has been referenced in:
  • Gao Z  et al. Male Men1 heterozygous mice exhibit fasting hyperglycemia in the early stage of MEN1. J Endocrinol 230:347-55 (2016). Read more (PubMed: 27432891) »
  • Long AJ  et al. Syk Inhibition Induces Platelet Dependent Peri-islet Hemorrhage in the Rat Pancreas. Toxicol Pathol 44:998-1012 (2016). Read more (PubMed: 27324990) »

See all 21 Publications for this product

Product Wall

Thank you for contacting us.

For ab10988: ICC/IF was added based on published references and a customer Abreview. Thus, a protocol would be based on these sources. We have not tested it ourselves in this application.

The abreview i...

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Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Syrian Hamster Tissue sections (pancreas)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate-buffer pH 6,0
Permeabilization No
Specification pancreas
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C
Fixative Formaldehyde
Username

Abcam user community

Verified customer

提交于 May 29 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rat Tissue sections (pancreas)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate-buffer pH 6,0
Permeabilization No
Specification pancreas
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C
Fixative Formaldehyde
Username

Abcam user community

Verified customer

提交于 May 28 2015

Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (pancreas)
Permeabilization No
Specification pancreas
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C
Fixative Formaldehyde
Username

Abcam user community

Verified customer

提交于 May 28 2015

Thank you for contacting us and your interest in our products. I am sorry for the delay in getting back to you in regards to the Anti-Glucagon antibody [K79bB10] (ab10988).

Glucagon is cleaved into 8 peptide chains one of them is GLP1. As this...

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Thank you for contacting us.

I have contacted the lab regarding the 4 antibodies you had inquired about, and will be in touch with more informaton as soon as I have it.

Thank you for your patience!


Use our products...

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Thank you for contacting us. I was able to find a paper describing production and characterization of the ab10988 clone, K79bB10, but as far as I can tell, the epitope has not been mapped. Here is a link to the paper's abstract: http://www.ncbi.n...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (pancreas)
Specification pancreas
Fixative Formaldehyde
Antigen retrieval step None
Permeabilization No
Blocking step (agent) for 10 minute(s) · Concentration: 100% · Temperature: RT°C
Username

Dr. Miri Stolovich-Rain

Verified customer

提交于 Nov 09 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rat Tissue sections (Pancreas)
Specification Pancreas
Fixative Paraformaldehyde
Antigen retrieval step None
Permeabilization No
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
Username

Mr. Thomas Schöppe

Verified customer

提交于 Jul 15 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Pancreas)
Specification Pancreas
Fixative Formaldehyde
Antigen retrieval step None
Permeabilization No
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C
Username

Abcam user community

Verified customer

提交于 Feb 10 2009

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"