1/1000 - 1/5000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Use at an assay dependent concentration.
Is unsuitable for Flow Cyt or IP.
Glucagon is a hormone that is secreted by alpha cells in the pancreas. Glucagon antagonizes insulin by converting glycogen to glucose in the liver and increasing blood sugar levels. Glucagon-like peptide 1 (GLP1), Glucagon-like peptide 2 (GLP2), VIP (vasoactive intestinal peptide) and PACAP (pituitary adenylate cyclase activating polypeptide) are in the glucagons hormone family. GLP1 is a transmitter in the central nervous system that regulates feeding and drinking behavior. GLP2 stimulates intestinal epithelial growth.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucagon antibody [EP3070] (ab92517)Image is courtesy of an anonymous AbReview.
Immunohistochemical analysis of PFA-fixed paraffin-embedded murine pancreatic tissue sections, labelling glucagon with ab92517 at a dilution of 1/2500 incubated for 18 hours at 4°C. Antigen retrival was performed through heat-mediated 10mM sodium citrate pH 6.0 incubation. Blocking was done with 5% serum for 1 hour at 25°C. The secondary was a donkey anti-rabbit polyclonal Alexa Fluor® 488 conjugate at 1/400. Counterstaining is with DAPI (blue) against nuclear DNA.
ab92517, at 1/1000 dilution, staining Glucagon in paraffin embedded Human pancreas tissue by Immunohistochemistry.
ELISA - Anti-Glucagon antibody [EP3070] (ab92517)
Direct ELISA antibody dose-response curve using recombinant anti-Human Glucagon antibody (ab92517) against recombinant human glucagon protein followed Alkaline Phosphatase conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) secondary antibody.
Antigen concentration: 1000 ng/ml
Primary ab concentration: 0-1000 ng/ml
Secondary ab concentration: 1:2500 dilution
Western blot - Anti-Glucagon antibody [EP3070] (ab92517)
All lanes : Anti-Glucagon antibody [EP3070] (ab92517)
Lane 1 : HepG2 Lysate Lane 2 : Human Fetal liver lysate Lane 3 : Mouse kidney lysate Lane 4 : Mouse spleen Lysate
Monazzam A et al. Increased Expression of GLP-1R in Proliferating Islets of Men1 Mice is Detectable by [68Ga]Ga-DO3A-VS-Cys40-Exendin-4 /PET. Sci Rep8:748 (2018).
Read more (PubMed: 29335487) »
Ortmann J et al. Immunohistochemical methods as an aid in estimating the time since death. Forensic Sci Int273:71-79 (2017).
Read more (PubMed: 28236731) »