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Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) (N terminal)
This product is a recombinant rabbit monoclonal antibody.
Alternative versions available:
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
Our Abpromise guarantee covers the use of ab92517 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 21 kDa.|
|IHC-P||1/1000 - 1/5000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|ELISA||Use at an assay dependent concentration.|
Immunohistochemical analysis of PFA-fixed paraffin-embedded murine pancreatic tissue sections, labelling glucagon with ab92517 at a dilution of 1/2500 incubated for 18 hours at 4°C. Antigen retrival was performed through heat-mediated 10mM sodium citrate pH 6.0 incubation. Blocking was done with 5% serum for 1 hour at 25°C. The secondary was a donkey anti-rabbit polyclonal Alexa Fluor® 488 conjugate at 1/400. Counterstaining is with DAPI (blue) against nuclear DNA.
Direct ELISA antibody dose-response curve using recombinant anti-Human Glucagon antibody (ab92517) against recombinant human glucagon protein followed Alkaline Phosphatase conjugated Goat Anti-Rabbit IgG secondary antibody.
Antigen concentration: 1000 ng/ml
Primary ab concentration: 0-1000 ng/ml
Secondary ab concentration: 1:2500 dilution