重组
RabMAb

Anti-Glucagon抗体[EP3070] (ab92517)

概述

  • 产品名称
    Anti-Glucagon抗体[EP3070]
    参阅全部 Glucagon 一抗
  • 描述
    兔单克隆抗体[EP3070] to Glucagon
  • 经测试应用
    适用于: WB, IHC-P, ELISAmore details
    不适用于: Flow Cyt or IP
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) (N terminal)

  • 阳性对照
    • HepG2, Human fetal liver, Mouse kidney and Mouse spleen lysates. Human pancreas tissue.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

     

    Alternative versions available:

    Anti-Glucagon antibody (BSA and Azide free) [EP3070] - (ab167078)

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

性能

应用

Our Abpromise guarantee covers the use of ab92517 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Predicted molecular weight: 21 kDa.
IHC-P 1/1000 - 1/5000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ELISA Use at an assay dependent concentration.
  • 应用说明
    Is unsuitable for Flow Cyt or IP.
  • 靶标

    • 相关性
      Glucagon is a hormone that is secreted by alpha cells in the pancreas. Glucagon antagonizes insulin by converting glycogen to glucose in the liver and increasing blood sugar levels. Glucagon-like peptide 1 (GLP1), Glucagon-like peptide 2 (GLP2), VIP (vasoactive intestinal peptide) and PACAP (pituitary adenylate cyclase activating polypeptide) are in the glucagons hormone family. GLP1 is a transmitter in the central nervous system that regulates feeding and drinking behavior. GLP2 stimulates intestinal epithelial growth.
    • 细胞定位
      Secreted
    • 数据库链接
    • 别名
      • GCG antibody

    Anti-Glucagon antibody [EP3070] 图像

    • Immunohistochemical analysis of PFA-fixed paraffin-embedded murine pancreatic tissue sections, labelling glucagon with ab92517 at a dilution of 1/2500 incubated for 18 hours at 4°C. Antigen retrival was performed through heat-mediated 10mM sodium citrate pH 6.0 incubation. Blocking was done with 5% serum for 1 hour at 25°C. The secondary was a donkey anti-rabbit polyclonal Alexa Fluor® 488 conjugate at 1/400. Counterstaining is with DAPI (blue) against nuclear DNA.

      See Abreview

    • Direct ELISA antibody dose-response curve using recombinant anti-Human Glucagon antibody (ab92517) against recombinant human glucagon protein followed Alkaline Phosphatase conjugated Goat Anti-Rabbit IgG secondary antibody.

      Antigen concentration: 1000 ng/ml

      Primary ab concentration: 0-1000 ng/ml

      Secondary ab concentration: 1:2500 dilution

    • ab92517, at 1/1000 dilution, staining Glucagon in paraffin embedded Human pancreas tissue by Immunohistochemistry.
    • All lanes : Anti-Glucagon antibody [EP3070] (ab92517)

      Lane 1 : HepG2 Lysate
      Lane 2 : Human Fetal liver lysate
      Lane 3 : Mouse kidney lysate
      Lane 4 : Mouse spleen Lysate


      Predicted band size : 21 kDa

    Anti-Glucagon antibody [EP3070] (ab92517)参考文献

    This product has been referenced in:
    • Ortmann J  et al. Immunohistochemical methods as an aid in estimating the time since death. Forensic Sci Int 273:71-79 (2017). IHC-P ; Human . Read more (PubMed: 28236731) »
    • Du W  et al. HID-1 is required for homotypic fusion of immature secretory granules during maturation. Elife 5:N/A (2016). WB ; Mouse . Read more (PubMed: 27751232) »

    See all 10 Publications for this product

    Product Wall

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (Pancreas)
    Permeabilization
    No
    Specification
    Pancreas
    Blocking step
    serum +1% BSA in 0.1% PBST as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    提交于 Mar 24 2017

    Application
    ELISA
    Sample
    Human Recombinant protein (Synthetic peptide)
    Specification
    Synthetic peptide
    Blocking step
    BSA as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
    Type
    Indirect
    Username

    Abcam user community

    Verified customer

    提交于 Mar 01 2016

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (pancreas)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: 10mm sodium citric PH 6.0
    Permeabilization
    No
    Specification
    pancreas
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    提交于 Sep 12 2015

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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