Anti-Glucagon抗体(Biotin) (ab48287)

概述

  • 产品名称Anti-Glucagon抗体(Biotin)
    参阅全部 Glucagon 一抗
  • 描述
    兔多克隆抗体to Glucagon (Biotin)
  • 偶联物Biotin
  • 经测试应用适用于: IP, ELISA, RIA, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Sheep, Rabbit, Hamster, Cow, Dog, Pig, Xenopus laevis
  • 免疫原

    Synthetic peptide:

    HSQGTFTSDYSKYLDSRRAQDFVQWLMNT

    conjugated to KLH via carboxyl group, corresponding to internal sequence amino acids 53-81 of Human Glucagon.

  • 阳性对照
    • This antibody gave a positive result in IHC in the following FFPE tissue: Human normal pancreas.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab48287 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IP Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.
RIA Use at an assay dependent concentration.
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

Anti-Glucagon antibody (Biotin) 图像

  • IHC image of Glucagon staining in THuman normal pancreas formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab48287, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

     

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-Glucagon antibody (Biotin) (ab48287)参考文献

ab48287 has not yet been referenced specifically in any publications.

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