概述

  • 产品名称Anti-Glucagon抗体
    参阅全部 Glucagon 一抗
  • 描述
    兔多克隆抗体to Glucagon
  • 特异性ab53704 detects endogenous levels of total Glucagon protein.
  • 经测试应用适用于: IHC-P, WB, ELISAmore details
  • 种属反应性
    与反应: Human, African Green Monkey
    预测可用于: Mouse, Rat
  • 免疫原

    Synthetic peptide derived from human Glucagon.

  • 阳性对照
    • COS7 cell extracts. IHC-P: Human pancreas FFPE tissue sections.

性能

应用

Our Abpromise guarantee covers the use of ab53704 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/500 - 1/1000. Detects a band of approximately 25 kDa (predicted molecular weight: 21 kDa).
ELISA 1/5000.

靶标

  • 相关性Glucagon is a hormone that is secreted by alpha cells in the pancreas. Glucagon antagonizes insulin by converting glycogen to glucose in the liver and increasing blood sugar levels. Glucagon-like peptide 1 (GLP1), Glucagon-like peptide 2 (GLP2), VIP (vasoactive intestinal peptide) and PACAP (pituitary adenylate cyclase activating polypeptide) are in the glucagons hormone family. GLP1 is a transmitter in the central nervous system that regulates feeding and drinking behavior. GLP2 stimulates intestinal epithelial growth.
  • 细胞定位Secreted
  • 数据库链接
  • 别名
    • GCG antibody

Anti-Glucagon antibody 图像

  • IHC image of Glucagon staining in human pancreas formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab53704, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • All lanes : Anti-Glucagon antibody (ab53704) at 1/500 dilution

    Lane 1 : COS7 cell extract
    Lane 2 : COS7 cell extract with immunising peptide


    Predicted band size : 21 kDa
    Observed band size : 25 kDa (why is the actual band size different from the predicted?)

Anti-Glucagon antibody (ab53704)参考文献

ab53704 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"