概述

  • 产品名称Anti-GLS2抗体
    参阅全部 GLS2 一抗
  • 描述
    兔多克隆抗体to GLS2
  • 经测试应用适用于: ICC/IF, IHC-Fr, WB, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to Human GLS2 (internal sequence).
    (Peptide available as ab169954)

  • 阳性对照
    • Rat kidney tissue lysate

性能

应用

Our Abpromise guarantee covers the use of ab113509 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 20 µg/ml.
IHC-Fr 1/1000.
WB Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 66 kDa.Can be blocked with GLS2 peptide (ab169954).
IHC-P Use a concentration of 5 µg/ml.

靶标

  • 功能Plays an important role in the regulation of glutamine catabolism. Promotes mitochondrial respiration and increases ATP generation in cells by catalyzing the synthesis of glutamate and alpha-ketoglutarate. Increases cellular anti-oxidant function via NADH and glutathione production. May play a role in preventing tumor proliferation.
  • 组织特异性Highly expressed in liver. Expressed in brain and pancreas. Not observed in heart, placenta, lung, skeletal muscle and kidney. Expression is significantly reduced in hepatocellular carcinomas.
  • 序列相似性Belongs to the glutaminase family.
    Contains 2 ANK repeats.
  • 细胞定位Mitochondrion.
  • Information by UniProt
  • 数据库链接
  • 别名
    • breast cell glutaminase antibody
    • GA antibody
    • GLS antibody
    • Gls2 antibody
    • GLSL_HUMAN antibody
    • glutaminase 2 (liver mitochondrial) antibody
    • glutaminase 2 antibody
    • glutaminase GA antibody
    • glutaminase I antibody
    • Glutaminase liver isoform antibody
    • hLGA antibody
    • L glutaminase antibody
    • L glutamine amidohydrolase antibody
    • L-glutaminase antibody
    • L-glutamine amidohydrolase antibody
    • LGA antibody
    • mitochondrial antibody
    • phosphate activated glutaminase antibody
    • phosphate-dependent glutaminase antibody
    see all

Anti-GLS2 antibody 图像

  • Ab113509 IHC-FoFr on rat brain tissue sections. The antibody was used at 1/1000 in the free floating technique for 18 hours at 20°C. There was no blocking step.

    See Abreview

  • Lane 1 : Anti-GLS2 antibody (ab113509) at 0.5 µg/ml
    Lane 2 : Anti-GLS2 antibody (ab113509) at 1 µg/ml

    Lane 1 : GLS2 in Rat Kidney Lysate
    Lane 2 : GLS2 in Rat Kidney Lysate


    Predicted band size : 66 kDa
  • Immunohistochemistry of GLS2 in human kidney tissue with ab113509 antibody at 5 ug/mL.
  • Immunofluorescence of GLS2 in Human Kidney cells using ab113509 at 20 ug/ml.

  • Immunocytochemistry/Immunofluorescence analysis of rat liver cells labelling GLS2 with ab113509 at 20 µg/mL. 

Anti-GLS2 antibody (ab113509)参考文献

This product has been referenced in:
  • Yu D  et al. Kidney-type glutaminase (GLS1) is a biomarker for pathologic diagnosis and prognosis of hepatocellular carcinoma. Oncotarget 6:7619-31 (2015). IHC ; Human . Read more (PubMed: 25844758) »
  • Brison E  et al. Novel treatment with neuroprotective and antiviral properties against a neuroinvasive human respiratory virus. J Virol 88:1548-63 (2014). Mouse . Read more (PubMed: 24227863) »

See all 2 Publications for this product

Product Wall

ab156876: Based on the immunogen sequence, this antibody is capable of detecting mouse kidney GLS1 isoforms 1 and 2 according to swissprot:

mouse GLS1: http://www.uniprot.org/uniprot/D3Z7P3

ab113509: I blasted human GLS2 against mouse...

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Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Antigen retrieval step None
Sample Rat Tissue sections (Brain)
Specification Brain
Permeabilization No
Fixative Paraformaldehyde
Username

Dr. Sophie Pezet

Verified customer

提交于 Jul 25 2013

For TIGAR, we recommend any of the lysates shown in the western blot on the datasheet. We do not, however, have free samples.

For GLS2, ab113509 was validated for western blotting on blots of rat kidney lysate. We have this as ab29480.
...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (liver)
Specification liver
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate buffer PH6
Permeabilization No
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Mr. Ahmed Ghallab

Verified customer

提交于 Apr 12 2012

Thank you for your reply.


Since we already replaced the antibody and that didin't work, we typically now will refund the original purchase. Please let me know if that is acceptable and I will refund your original order for ab91073.
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Thank you for your reply.

Can you please provide your previous order number for ab113509 and I will arrange for the replacement?


I look forward to your reply so that I may assist you further.

Thank you for your reply. I am happy to hear you were able to obtain the expected results from this antibody.

In order to share your results with us I would please ask you to submit an Abreview. To do it, please visit the site:

http...

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Thank you for contacting us.

In order to reduce the background signal, there are a few suggestions that may help:

-Perform an optimal fixation process, using 10%NBF between 12-24 hours.

-The tissue may be over retrieved. T...

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Thank you for your reply.


I am not sure I have any additional protocol suggestions to make that would improve your results. It is possible that the antibody is picking up some non-specific bands in addition to GLS2. I am happy to provid...

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Thank you for your patience as I have further investigated your inquiry.


The exact sequence of the immunogen is proprietary.

Regarding your protocol, the main modification I would recommend would be to switch from BSA to milk...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"