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Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human GLP1 aa 50-150.
Database link: P01275
This product is a recombinant rabbit monoclonal antibody.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our Abpromise guarantee covers the use of ab108443 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use a concentration of 0.5 - 1 µg/ml.|
|WB||1/2000. Predicted molecular weight: 21 kDa.
For unpurified, use 1/200.
|IHC-P||1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
1 ug/mL of Glucagon-like Peptide (GLP-1) was coated into 96-wells. Various concentrations of anti-Glucagon-Like Peptide 1 (GLP-1, 7-36,amide) rabbit monoclonal antibody (unpurified ab108443) was added. HRP conjugated goat anti-rabbit IgG antibody was used to develop the color.
Unpurified ab108443, at 1/100 dilution, staining Glicentin in Human pancreas tissue by immunohistochemistry
Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
ab108443 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"