概述

  • 产品名称
  • 描述
    兔多克隆抗体to GLP1
  • 经测试应用
    适用于: IHC-Fr, IHC-P, IHC-R, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
    预测可用于: Mammal
  • 免疫原

    Synthetic peptide corresponding to Human GLP1 aa 1-19 conjugated to Bovine Serum Albumin (BSA) (Bis-diazotised tolidine (Bdt)). The mid to C terminal region of GLP 1 [1-19] enabling immunoreactivity with N terminal truncated and C terminally extended forms of GLP 1 (e.g. GLP 1[7 to 36]amide).
    Sequence:

    HDEFERHAEGTFTSDVSSY

  • 阳性对照
    • Mammalian endocrine pancreas (A cells) and colon (L/EG cells) are heavily labelled with this antiserum.

应用

Our Abpromise guarantee covers the use of ab22625 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-Fr Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
IHC-R Use at an assay dependent concentration.
Vibratome slices, All standard crosslinking agents may be used. 1/500 to 1/4000, using overnight incubation and peroxidase anti peroxidase procedure. 1/100 to 1/1000, using overnight incubation and indirect immunofluorescence procedure.
ICC/IF Use at an assay dependent concentration. Used at a dilution of 1/500 for 2 hrs using mouse small intestinal cells (see Abreview for further details).

靶标

  • 功能
    Glucagon plays a key role in glucose metabolism and homeostasis. Regulates blood glucose by increasing gluconeogenesis and decreasing glycolysis. A counterregulatory hormone of insulin, raises plasma glucose levels in response to insulin-induced hypoglycemia. Plays an important role in initiating and maintaining hyperglycemic conditions in diabetes.
    GLP-1 is a potent stimulator of glucose-dependent insulin release. Play important roles on gastric motility and the suppression of plasma glucagon levels. May be involved in the suppression of satiety and stimulation of glucose disposal in peripheral tissues, independent of the actions of insulin. Have growth-promoting activities on intestinal epithelium. May also regulate the hypothalamic pituitary axis (HPA) via effects on LH, TSH, CRH, oxytocin, and vasopressin secretion. Increases islet mass through stimulation of islet neogenesis and pancreatic beta cell proliferaton. Inhibits beta cell apoptosis.
    GLP-2 stimulates intestinal growth and up-regulates villus height in the small intestine, concomitant with increased crypt cell proliferation and decreased enterocyte apoptosis. The gastrointestinal tract, from the stomach to the colon is the principal target for GLP-2 action. Plays a key role in nutrient homeostasis, enhancing nutrient assimilation through enhanced gastrointestinal function, as well as increasing nutrient disposal. Stimulates intestinal glucose transport and decreases mucosal permeability.
    Oxyntomodulin significantly reduces food intake. Inhibits gastric emptying in humans. Suppression of gastric emptying may lead to increased gastric distension, which may contribute to satiety by causing a sensation of fullness.
    Glicentin may modulate gastric acid secretion and the gastro-pyloro-duodenal activity. May play an important role in intestinal mucosal growth in the early period of life.
  • 组织特异性
    Glucagon is secreted in the A cells of the islets of Langerhans. GLP-1, GLP-2, oxyntomodulin and glicentin are secreted from enteroendocrine cells throughout the gastrointestinal tract. GLP1 and GLP2 are also secreted in selected neurons in the brain.
  • 序列相似性
    Belongs to the glucagon family.
  • 翻译后修饰
    Proglucagon is post-translationally processed in a tissue-specific manner in pancreatic A cells and intestinal L cells. In pancreatic A cells, the major bioactive hormone is glucagon cleaved by PCSK2/PC2. In the intestinal L cells PCSK1/PC1 liberates GLP-1, GLP-2, glicentin and oxyntomodulin. GLP-1 is further N-terminally truncated by post-translational processing in the intestinal L cells resulting in GLP-1(7-37) GLP-1-(7-36)amide. The C-terminal amidation is neither important for the metabolism of GLP-1 nor for its effects on the endocrine pancreas.
  • 细胞定位
    Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • GCG antibody
    • glicentin-related polypeptide antibody
    • GLP-1 antibody
    • GLP-1(7-36) antibody
    • GLP-1(7-37) antibody
    • GLP-2 antibody
    • GLP1 antibody
    • GLP1, included antibody
    • GLP2 antibody
    • GLP2, included antibody
    • GLUC_HUMAN antibody
    • Glucagon antibody
    • Glucagon like peptide 1 antibody
    • glucagon-like peptide 1 antibody
    • Glucagon-like peptide 1, included antibody
    • Glucagon-like peptide 2 antibody
    • Glucagon-like peptide 2, included antibody
    • GRPP antibody
    • OXM antibody
    • OXY antibody
    • preproglucagon antibody
    see all

Anti-GLP1 antibody 图像

  • ab22625 (1/2000) staining GLP1 in human pancreas using an automated system (DAKO Autostainer Plus). Using this protocol there is strong cytoplasmic staining in the pancreatic islets.
    Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
  • ab22625 at a 1/500 dilution staining mouse small intestine (pancreas) by ICC/IF. The cells were paraformaldehyde fixed and blocked with 5% serum prior to incubation with the antibody for 2 hours. Bound antibody was detected using a Cy2 conjugated donkey anti-rabbit IgG (H+L). As expected, the small intestine staining is in a very low number of entero-endocrine cells (L-cells), laying on the instestinal villi.

    This image is courtesy of an Abreview.

    See Abreview

Anti-GLP1 antibody (ab22625)参考文献

This product has been referenced in:
  • Liu L  et al. Chromanol 293B, an inhibitor of KCNQ1 channels, enhances glucose-stimulated insulin secretion and increases glucagon-like peptide-1 level in mice. Islets 6:e962386 (2014). Read more (PubMed: 25437377) »
  • Duca FA  et al. Combination of obesity and high-fat feeding diminishes sensitivity to GLP-1R agonist exendin-4. Diabetes 62:2410-5 (2013). IF . Read more (PubMed: 23423571) »

See all 6 Publications for this product

Product Wall

Thank you for your enquiry.

I can confirm that this particular productis sourced externally, and regrettably they do not have any specific data or information regarding in house testing for cross reactivity with analogue liraglutide (Lys(&ga...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Intestine)
Specification
Intestine
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% triton x-100
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Username

Abcam user community

Verified customer

提交于 Mar 26 2012

Thank you for contacting us.

ab26278 would work with Mouse tissue and in IHC-Fr. Please be advised that this is a primary antibody not the secondary antibody. The compatible FITC conjugated secondary antibodies are

ab97239, ab98692...

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Thank you for your enquiry.

I am sorry I am not able to find catalog number 1072-1002 on our system. Our product numbers always start with ab. We do have the following goat anti mouse IgG FITC conjugated secondary antibody, tested in IHC-Fr,...

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Thats great news. I have generated a discount code for you:

DISCOUNT CODE: xxxxx
Expiration date: 2012-06-19

As discussed this code will give you 1 freeprimary antibodybefore the expiration date. To redeem this offer, please su...

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Thank you for that extra information.

Having gone through the protocol and the additional information about the technique you have provided, I would suggest trying anti-GLP1 antibody, http://www.abcam.com/GLP1-antibody-ab22625.html. This is a...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Pancreas)
Specification
Pancreas
Fixative
10% Formalin
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate buffer
Permeabilization
No
Blocking step
Serum as blocking agent for 10 minute(s) · Concentration: 100%
Username

Abcam user community

Verified customer

提交于 Sep 21 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Duodenum)
Specification
Duodenum
Fixative
Paraformaldehyde
Antigen retrieval step
None
Permeabilization
No
Blocking step
Serum as blocking agent for 40 minute(s) · Concentration: 5% · Temperature: 24°C
Username

Abcam user community

Verified customer

提交于 Feb 03 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Small Intestine)
Specification
Small Intestine
Fixative
Paraformaldehyde
Permeabilization
Yes - TBST
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: RT°C
Username

Abcam user community

Verified customer

提交于 Jan 11 2011

See the UniProt page for GLP1 molecular processing: http://www.uniprot.org/uniprot/P01275 Different peptides are cleaved from Glucagon, including: Glicentin Oxyntomodulin Glucagon Glucagon-like peptide 1 Glucagon-like peptide 1 (7-37) Glucago...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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