概述

  • 产品名称
    Anti-GLP1抗体[9F8]
    参阅全部 GLP1 一抗
  • 描述
    小鼠单克隆抗体[9F8] to GLP1
  • 特异性
    ab23447 recognises low activity enzyme GLP1. No crossreactitvity with either activated forms GLP1 (7-36) amide or GLP1 (7-37) amide.
  • 经测试应用
    适用于: ELISA, WB, IHC-Frmore details
  • 种属反应性
    As GLP1 is identical across all mammals studied the antibody is expected to cross-react with GLP1 from all mammals.
  • 免疫原

    Synthetic peptide coupled to diphtheria toxoid with glutaraldehyde.

  • 表位
    The epitope is located in the region of residues 1-6 of GLP1.

性能

应用

Our Abpromise guarantee covers the use of ab23447 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/16000.
WB Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.

靶标

  • 功能
    Glucagon plays a key role in glucose metabolism and homeostasis. Regulates blood glucose by increasing gluconeogenesis and decreasing glycolysis. A counterregulatory hormone of insulin, raises plasma glucose levels in response to insulin-induced hypoglycemia. Plays an important role in initiating and maintaining hyperglycemic conditions in diabetes.
    GLP-1 is a potent stimulator of glucose-dependent insulin release. Play important roles on gastric motility and the suppression of plasma glucagon levels. May be involved in the suppression of satiety and stimulation of glucose disposal in peripheral tissues, independent of the actions of insulin. Have growth-promoting activities on intestinal epithelium. May also regulate the hypothalamic pituitary axis (HPA) via effects on LH, TSH, CRH, oxytocin, and vasopressin secretion. Increases islet mass through stimulation of islet neogenesis and pancreatic beta cell proliferaton. Inhibits beta cell apoptosis.
    GLP-2 stimulates intestinal growth and up-regulates villus height in the small intestine, concomitant with increased crypt cell proliferation and decreased enterocyte apoptosis. The gastrointestinal tract, from the stomach to the colon is the principal target for GLP-2 action. Plays a key role in nutrient homeostasis, enhancing nutrient assimilation through enhanced gastrointestinal function, as well as increasing nutrient disposal. Stimulates intestinal glucose transport and decreases mucosal permeability.
    Oxyntomodulin significantly reduces food intake. Inhibits gastric emptying in humans. Suppression of gastric emptying may lead to increased gastric distension, which may contribute to satiety by causing a sensation of fullness.
    Glicentin may modulate gastric acid secretion and the gastro-pyloro-duodenal activity. May play an important role in intestinal mucosal growth in the early period of life.
  • 组织特异性
    Glucagon is secreted in the A cells of the islets of Langerhans. GLP-1, GLP-2, oxyntomodulin and glicentin are secreted from enteroendocrine cells throughout the gastrointestinal tract. GLP1 and GLP2 are also secreted in selected neurons in the brain.
  • 序列相似性
    Belongs to the glucagon family.
  • 翻译后修饰
    Proglucagon is post-translationally processed in a tissue-specific manner in pancreatic A cells and intestinal L cells. In pancreatic A cells, the major bioactive hormone is glucagon cleaved by PCSK2/PC2. In the intestinal L cells PCSK1/PC1 liberates GLP-1, GLP-2, glicentin and oxyntomodulin. GLP-1 is further N-terminally truncated by post-translational processing in the intestinal L cells resulting in GLP-1(7-37) GLP-1-(7-36)amide. The C-terminal amidation is neither important for the metabolism of GLP-1 nor for its effects on the endocrine pancreas.
  • 细胞定位
    Secreted.
  • Information by UniProt
  • 别名
    • GCG antibody
    • glicentin-related polypeptide antibody
    • GLP-1 antibody
    • GLP-1(7-36) antibody
    • GLP-1(7-37) antibody
    • GLP-2 antibody
    • GLP1 antibody
    • GLP1, included antibody
    • GLP2 antibody
    • GLP2, included antibody
    • GLUC_HUMAN antibody
    • Glucagon antibody
    • Glucagon like peptide 1 antibody
    • glucagon-like peptide 1 antibody
    • Glucagon-like peptide 1, included antibody
    • Glucagon-like peptide 2 antibody
    • Glucagon-like peptide 2, included antibody
    • GRPP antibody
    • OXM antibody
    • OXY antibody
    • preproglucagon antibody
    see all

图片

  • ab23447 at a 1/500 dilution staining GLP1 from frozen sections of human GI tract by immunohistochemistry. An HRP conjugated anti-mouse antibody was used as the secondary reagent.

    This image is courtesy of an Abreview submitted by an anonymous researcher on 8 December 2005.

    See Abreview

文献

This product has been referenced in:
  • Duan FF  et al. Engineered commensal bacteria reprogram intestinal cells into glucose-responsive insulin-secreting cells for the treatment of diabetes. Diabetes 64:1794-803 (2015). IF . Read more (PubMed: 25626737) »

See 1 Publication for this product

客户评价及客户问答

Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (GI tract)
Specification
GI tract
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 1
Username

Abcam user community

Verified customer

提交于 Feb 08 2006

Thank you for your enquiry. The concentration of this antibody is 1 mg/mL. Should you require further assistance, please do not hesitate to contact me.

Thank you for your enquiry. ab23447 is specific for the low activity enzyme GLP-1(1-36)amide, but not with either of the activated forms GLP-1(7-36)amide and GLP-1(7-37)amide. You may find the paper below useful for further information on in the ...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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