概述

  • 产品名称Anti-GLP1抗体[11E2]
    参阅全部 GLP1 一抗
  • 描述
    小鼠单克隆抗体[11E2] to GLP1
  • 特异性This antibody reacts with all forms of GLP 1, including precursor.
  • 经测试应用适用于: ICC/IF, ELISA, WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
    预测可用于: Guinea pig, Cow
  • 免疫原

    Synthetic amide peptide:

    VAGLFVMLVQGSWQQDTEEKSRSLRSFSAS

    , corresponding to amino acids 7-36 of GLP1, coupled to carrier and adsorbed onto aluminum hydroxide gel

  • 表位This antibody reacts with a mid-molecular epitope of GLP 1.

性能

应用

Our Abpromise guarantee covers the use of ab23468 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use at an assay dependent dilution. PubMed: 20163204
ELISA 1/20000.
WB 1/1000. Predicted molecular weight: 21 kDa.

靶标

  • 功能Glucagon plays a key role in glucose metabolism and homeostasis. Regulates blood glucose by increasing gluconeogenesis and decreasing glycolysis. A counterregulatory hormone of insulin, raises plasma glucose levels in response to insulin-induced hypoglycemia. Plays an important role in initiating and maintaining hyperglycemic conditions in diabetes.
    GLP-1 is a potent stimulator of glucose-dependent insulin release. Play important roles on gastric motility and the suppression of plasma glucagon levels. May be involved in the suppression of satiety and stimulation of glucose disposal in peripheral tissues, independent of the actions of insulin. Have growth-promoting activities on intestinal epithelium. May also regulate the hypothalamic pituitary axis (HPA) via effects on LH, TSH, CRH, oxytocin, and vasopressin secretion. Increases islet mass through stimulation of islet neogenesis and pancreatic beta cell proliferaton. Inhibits beta cell apoptosis.
    GLP-2 stimulates intestinal growth and up-regulates villus height in the small intestine, concomitant with increased crypt cell proliferation and decreased enterocyte apoptosis. The gastrointestinal tract, from the stomach to the colon is the principal target for GLP-2 action. Plays a key role in nutrient homeostasis, enhancing nutrient assimilation through enhanced gastrointestinal function, as well as increasing nutrient disposal. Stimulates intestinal glucose transport and decreases mucosal permeability.
    Oxyntomodulin significantly reduces food intake. Inhibits gastric emptying in humans. Suppression of gastric emptying may lead to increased gastric distension, which may contribute to satiety by causing a sensation of fullness.
    Glicentin may modulate gastric acid secretion and the gastro-pyloro-duodenal activity. May play an important role in intestinal mucosal growth in the early period of life.
  • 组织特异性Glucagon is secreted in the A cells of the islets of Langerhans. GLP-1, GLP-2, oxyntomodulin and glicentin are secreted from enteroendocrine cells throughout the gastrointestinal tract. GLP1 and GLP2 are also secreted in selected neurons in the brain.
  • 序列相似性Belongs to the glucagon family.
  • 翻译后修饰Proglucagon is post-translationally processed in a tissue-specific manner in pancreatic A cells and intestinal L cells. In pancreatic A cells, the major bioactive hormone is glucagon cleaved by PCSK2/PC2. In the intestinal L cells PCSK1/PC1 liberates GLP-1, GLP-2, glicentin and oxyntomodulin. GLP-1 is further N-terminally truncated by post-translational processing in the intestinal L cells resulting in GLP-1(7-37) GLP-1-(7-36)amide. The C-terminal amidation is neither important for the metabolism of GLP-1 nor for its effects on the endocrine pancreas.
  • 细胞定位Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • GCG antibody
    • glicentin-related polypeptide antibody
    • GLP-1 antibody
    • GLP-1(7-36) antibody
    • GLP-1(7-37) antibody
    • GLP-2 antibody
    • GLP1 antibody
    • GLP1, included antibody
    • GLP2 antibody
    • GLP2, included antibody
    • GLUC_HUMAN antibody
    • Glucagon antibody
    • Glucagon like peptide 1 antibody
    • glucagon-like peptide 1 antibody
    • Glucagon-like peptide 1, included antibody
    • Glucagon-like peptide 2 antibody
    • Glucagon-like peptide 2, included antibody
    • GRPP antibody
    • OXM antibody
    • OXY antibody
    • preproglucagon antibody
    see all

Anti-GLP1 antibody [11E2] 图像

  • ICC/IF image of ab23468 stained PC12 cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab23468, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879 Dylight 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-GLP1 antibody [11E2] (ab23468)参考文献

This product has been referenced in:
  • Takada Y  et al. Brg1 plays an essential role in development and homeostasis of the duodenum through regulation of Notch signaling. Development 143:3532-3539 (2016). Read more (PubMed: 27510977) »
  • Marques C  et al. Sitagliptin prevents inflammation and apoptotic cell death in the kidney of type 2 diabetic animals. Mediators Inflamm 2014:538737 (2014). WB ; Rat . Read more (PubMed: 24817793) »

See all 4 Publications for this product

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Thank you for contacting us. Indeed, we do not have an antibody tested for all the applications/species. We can however provide you with a testing discount if you want to test one of these antibodies against an untested species or application. ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"