Anti-GLP-1抗体[8G9] (ab26278)
Key features and details
- Mouse monoclonal [8G9] to GLP-1
- Suitable for: IHC-P, Sandwich ELISA
- Reacts with: Mouse, Human
- Isotype: IgG1
概述
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产品名称
Anti-GLP-1抗体[8G9]
参阅全部 GLP-1 一抗 -
描述
小鼠单克隆抗体[8G9] to GLP-1 -
宿主
Mouse -
经测试应用
适用于: IHC-P, Sandwich ELISAmore details -
种属反应性
与反应: Mouse, Human
预测可用于: a wide range of other species -
免疫原
Synthetic peptide corresponding to Human GLP-1 aa 50-150. ab26278 reacts with the amidated C-Terminus of GLP-1(7-36) coupled to a carrier.
Database link: P01275 -
表位
C-terminal epitope of GLP-1(7-36)amide -
阳性对照
- IHC-P/IHC-Fr: rat colon, rat and mouse pancreas tissue.
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常规说明
ab26278 should be used as capture with biotinylated version ab121057 as detection.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.097% Sodium azide
Constituents: 0.0268% PBS, 2.9% Sodium chloride -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
8G9 -
骨髓瘤
x63-Ag8.653 -
同种型
IgG1 -
轻链类型
kappa -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
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sELISA pair antibody
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab26278于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-P |
1/2000.
Fix in 4% paraformaldehyde in 0.1 phosphate buffer, pH 7.4 overnight at 4°C. |
|
Sandwich ELISA |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Mouse monoclonal [10] to GLP-1 (ab121057) and Mouse monoclonal [4F3] to GLP-1 (ab23472).
|
说明 |
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IHC-P
1/2000. Fix in 4% paraformaldehyde in 0.1 phosphate buffer, pH 7.4 overnight at 4°C. |
Sandwich ELISA
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Mouse monoclonal [10] to GLP-1 (ab121057) and Mouse monoclonal [4F3] to GLP-1 (ab23472). |
靶标
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功能
Glucagon plays a key role in glucose metabolism and homeostasis. Regulates blood glucose by increasing gluconeogenesis and decreasing glycolysis. A counterregulatory hormone of insulin, raises plasma glucose levels in response to insulin-induced hypoglycemia. Plays an important role in initiating and maintaining hyperglycemic conditions in diabetes.
GLP-1 is a potent stimulator of glucose-dependent insulin release. Play important roles on gastric motility and the suppression of plasma glucagon levels. May be involved in the suppression of satiety and stimulation of glucose disposal in peripheral tissues, independent of the actions of insulin. Have growth-promoting activities on intestinal epithelium. May also regulate the hypothalamic pituitary axis (HPA) via effects on LH, TSH, CRH, oxytocin, and vasopressin secretion. Increases islet mass through stimulation of islet neogenesis and pancreatic beta cell proliferaton. Inhibits beta cell apoptosis.
GLP-2 stimulates intestinal growth and up-regulates villus height in the small intestine, concomitant with increased crypt cell proliferation and decreased enterocyte apoptosis. The gastrointestinal tract, from the stomach to the colon is the principal target for GLP-2 action. Plays a key role in nutrient homeostasis, enhancing nutrient assimilation through enhanced gastrointestinal function, as well as increasing nutrient disposal. Stimulates intestinal glucose transport and decreases mucosal permeability.
Oxyntomodulin significantly reduces food intake. Inhibits gastric emptying in humans. Suppression of gastric emptying may lead to increased gastric distension, which may contribute to satiety by causing a sensation of fullness.
Glicentin may modulate gastric acid secretion and the gastro-pyloro-duodenal activity. May play an important role in intestinal mucosal growth in the early period of life. -
组织特异性
Glucagon is secreted in the A cells of the islets of Langerhans. GLP-1, GLP-2, oxyntomodulin and glicentin are secreted from enteroendocrine cells throughout the gastrointestinal tract. GLP1 and GLP2 are also secreted in selected neurons in the brain. -
序列相似性
Belongs to the glucagon family. -
翻译后修饰
Proglucagon is post-translationally processed in a tissue-specific manner in pancreatic A cells and intestinal L cells. In pancreatic A cells, the major bioactive hormone is glucagon cleaved by PCSK2/PC2. In the intestinal L cells PCSK1/PC1 liberates GLP-1, GLP-2, glicentin and oxyntomodulin. GLP-1 is further N-terminally truncated by post-translational processing in the intestinal L cells resulting in GLP-1(7-37) GLP-1-(7-36)amide. The C-terminal amidation is neither important for the metabolism of GLP-1 nor for its effects on the endocrine pancreas. -
细胞定位
Secreted. - Information by UniProt
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数据库链接
- Entrez Gene: 2641 Human
- Entrez Gene: 14526 Mouse
- Omim: 138030 Human
- SwissProt: P01275 Human
- SwissProt: P55095 Mouse
- Unigene: 516494 Human
- Unigene: 45494 Mouse
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别名
- GCG antibody
- Glicentin related polypeptide antibody
- glicentin-related polypeptide antibody
see all
图片
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Sandwich ELISA graph showing GLP-1 (1-36)amide, GLP-1 (9-36)amide, and GLP-1 (7-36)amide detection using ab26278 as capture antibody and biotinylated ab23468 detection antibody.
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ab26278 (1µg/ml) staining GLP-1 in Human pancreas, using an automated system (DAKO Autostainer Plus). Using this protocol there is strong cytoplasmic staining.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer citrate pH6.1 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required. -
Immunofluorescence analysis of diabetic mice pancreas tissue stained for GLP-1 using ab26278 at 1/500 dilution.
数据表及文件
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SDS download
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Datasheet download
文献 (20)
ab26278 被引用在 20 文献中.
- Cabello-Olmo M et al. Antidiabetic Effects of Pediococcus acidilactici pA1c on HFD-Induced Mice. Nutrients 14:N/A (2022). PubMed: 35277051
- Aerts L et al. Novel Homozygous Inactivating Mutation in the PCSK1 Gene in an Infant with Congenital Malabsorptive Diarrhea. Genes (Basel) 12:N/A (2021). PubMed: 34068683
- Dang JT et al. Ileal microbial shifts after Roux-en-Y gastric bypass orchestrate changes in glucose metabolism through modulation of bile acids and L-cell adaptation. Sci Rep 11:23813 (2021). PubMed: 34893681
- Kondo T et al. The Amount of Residual Incretin Regulates the Pancreatic ß-cell Function and Glucose Homeostasis. Intern Med 60:1433-1442 (2021). PubMed: 33952814
- Grunddal KV et al. Selective release of gastrointestinal hormones induced by an orally active GPR39 agonist. Mol Metab 49:101207 (2021). PubMed: 33711555