Recombinant protein fragment corresponding to a region within amino acids 1 and 171 of GLO1 (NP_006699).
WB: 293T, A431, H1299, HeLaS3, HepG2, MOLT and Raji cell lysates
IHC-P: ovarian carcinoma tissue
ICC/IF: HeLa cells
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol, 0.1M Tris, 0.1M Glycine, pH 7
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Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/3000. Predicted molecular weight: 21 kDa.
1/100 - 1/250.
1/100 - 1/200.
Catalyzes the conversion of hemimercaptal, formed from methylglyoxal and glutathione, to S-lactoylglutathione.
Secondary metabolite metabolism; methylglyoxal degradation; (R)-lactate from methylglyoxal: step 1/2.
Belongs to the glyoxalase I family.
Information by UniProt
Anti-GLO1 antibody 图像
Western blot - GLO1 antibody (ab96032)
All lanes : Anti-GLO1 antibody (ab96032) at 1/1000 dilution Lane 1 : 293T whole cell lysate Lane 2 : A431 whole cell lysate Lane 3 : H1299 whole cell lysate Lane 4 : HeLa whole cell lysate Lane 5 : HepG2 whole cell lysate Lane 6 : MOLT4 whole cell lysate Lane 7 : Raji whole cell lysate Lysates/proteins at 30 µg per lane. Predicted band size : 21 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - GLO1 antibody (ab96032)
Immunohistochemical analysis of GLO1 in paraffin embedded ovarian carcinoma tissue, using ab96032 at a 1/100 dilution.
Immunocytochemistry/ Immunofluorescence - GLO1 antibody (ab96032)
Immunofluorescence analysis of GLO1 in paraformaldehyde fixed HeLa, using ab96032 at a 1/200 dilution. Lower image: merged with DNA probe.
Anti-GLO1 antibody (ab96032)参考文献
This product has been referenced in:
et al. Methylglyoxal-induced glycation changes adipose tissue vascular architecture, flow and expansion, leading to insulin resistance. Sci Rep 7:1698 (2017).
Read more (PubMed: 28490763) »
et al. A Glyoxalase-1 Knockdown Does Not Have Major Short Term Effects on Energy Expenditure and Atherosclerosis in Mice. J Diabetes Res 2016:2981639 (2016).
Read more (PubMed: 26788517) »
See all 6 Publications
for this product
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"