Anti-GLIS3抗体(ab51268)

概述

  • 产品名称
  • 描述
    兔多克隆抗体to GLIS3
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Dog
  • 免疫原

    A region within synthetic peptide: MMVQRLGLIS PPASQVSTAC NQISPSLQRA MNAANLNIPP SDTRSLISRE S, corresponding to N terminal amino acids 1-50 of Human GLIS3

  • 阳性对照
    • HepG2 cell lyaste.

性能

应用

Our Abpromise guarantee covers the use of ab51268 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 2.5 µg/ml. Predicted molecular weight: 84 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 相关性
    GLIS3 is a member of the GLI similar zinc finger protein family, and encodes a nuclear protein with five C2H2 type zinc finger domains. It functions as both an activator and repressor of transcription, and is specifically involved in the development of pancreatic beta cells, thyroid, eye, liver and kidney. Mutations in this gene have been associated with neonatal diabetes and congenital hypothyroidism (NDH). Alternatively spliced variants that encode different protein isoforms have been described but the full length nature of only two have been determined.
  • 细胞定位
    Nuclear
  • 数据库链接
  • 别名
    • FLJ38999 antibody
    • FLJ90578 antibody
    • GLI similar 3 antibody
    • GLI SIMILAR PROTEIN 3 antibody
    • GLIS family zinc finger 3 antibody
    • MGC33662 antibody
    • OK/KNS-cl.4 antibody
    • Zinc finger protein 515 antibody
    • Zinc finger protein GLIS3 antibody
    • ZNF515 antibody
    see all

实验方案

文献

ab51268 has not yet been referenced specifically in any publications.

客户评价及客户问答


Having reviewed this case, I would like to offer some suggestions to help optimize the results from ab51268. I would also appreciate if you can confirm some further details:
1. Have the cells you have been using been frequently passaged? If s...

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Thank you very much for your interest in ab51268.

We do not currently have an image of ab51268 in WB. Therefore, I can offer a discount off a future purchase if you buy ab51268 now, use it WB and submit an image of your results to us in the f...

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Application
Western blot
Sample
Rat Tissue lysate - whole (PANCREAS)
Loading amount
30 µg
Specification
PANCREAS
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

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Verified customer

提交于 Jul 08 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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