Anti-GliS2抗体(ab28462)

概述

  • 产品名称
    Anti-GliS2抗体
  • 描述
    兔多克隆抗体to GliS2
  • 特异性
    ab28462 recognises GLIS2.
  • 经测试应用
    适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Chicken, Cow, Dog
  • 免疫原

    Synthetic peptide derived from residues 183 - 232 of human GliS2.

    .

  • 阳性对照
    • HepG2 cell lysates

性能

应用

Our Abpromise guarantee covers the use of ab28462 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.5 µg/ml. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA 1/312500.

靶标

  • 功能
    Can act either as a transcription repressor or as a transcription activator, depending on the cell context. Represses the transcriptional activation mediated by CTNNB1 in the Wnt pathway. May act by recruiting the corepressors CTBP1 and HDAC3. May be involved in neuron differentiation.
  • 组织特异性
    Expressed at high levels in kidney and at low levels in heart, lung and placenta. Expressed in colon.
  • 疾病相关
    Defects in GLIS2 are the cause of nephronophthisis type 7 (NPHP7) [MIM:611498]. NPHP7 is an autosomal recessive disorder resulting in end-stage renal disease during childhood or adolescence. It is a progressive tubulo-interstitial kidney disorder histologically characterized by modifications of the tubules with thickening of the basement membrane, interstitial fibrosis and, in the advanced stages, medullary cysts.
  • 序列相似性
    Belongs to the GLI C2H2-type zinc-finger protein family.
    Contains 5 C2H2-type zinc fingers.
  • 结构域
    The C2H2-type zinc finger 1 has a major repressor function and is required for CTNNB1 binding.
  • 翻译后修饰
    C-terminus cleavage is induced by interaction with CTNND1 and enhanced by Src tyrosine kinase.
  • 细胞定位
    Nucleus speckle. Cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • FLJ38247 antibody
    • GLI similar 2 antibody
    • GLI-similar 2 antibody
    • GLIS 2 antibody
    • GLIS family zinc finger 2 antibody
    • glis2 antibody
    • GLIS2_HUMAN antibody
    • Kruppel like zinc finger protein GLIS2 antibody
    • Neuronal Krueppel-like protein antibody
    • Zinc finger protein GLIS2 antibody
    see all

图片

  • Anti-GliS2 antibody (ab28462) at 0.5 µg/ml

    Predicted band size : 56 kDa
    Observed band size : 56 kDa

实验方案

文献

ab28462 has not yet been referenced specifically in any publications.

客户评价及客户问答

Thank you for your enquiry. The difference between ab48861 and ab28462 can be found in the purity and concentration. I have listed the differences below and some references that might be useful to you. ab48861 purity: protein A purified concent...

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