ELISA: Use at an assay dependent dilution. Antibody detection limit dilution 1/64000.
WB: Use at an assay dependent dilution. Predicted molecular weight: 30 kDa. Preliminary experiments gave an approx 40 kDa band in human prostate lysates after 1 µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 30.4 kDa according to NP_006842.2. The ~40 kDa band was successfully blocked by incubation with the immunizing peptide.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
According to PubMed:8973356, it is ubiquitously expressed with high levels in lung and kidney and low levels in heart and liver. Highly expressed in cell lines derived from nervous system tumors arising from glia, low or absent in non-glial-derived nervous system tumor cell lines. Also found in fetal kidney. According to PubMed:7607567 it is expressed only in brain tumor glioblastoma multiforme/astrocytoma and not in other nervous system tumors or normal fetal or adult tissues.