Anti-GLIPR1抗体(ab118382)

概述

  • 产品名称Anti-GLIPR1抗体
    参阅全部 GLIPR1 一抗
  • 描述
    小鼠单克隆抗体to GLIPR1
  • 经测试应用适用于: WB, ELISA, Flow Cytmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant fragment, corresponding to amino acids 23-100 of Human GLIPR1 with proprietary tag (NP_006842).

  • 阳性对照
    • HeLa whole cell lysate.

性能

应用

Our Abpromise guarantee covers the use of ab118382 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 30 kDa.
ELISA Use at an assay dependent concentration.
Flow Cyt Use 0.1µg for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

靶标

  • 组织特异性According to PubMed:8973356, it is ubiquitously expressed with high levels in lung and kidney and low levels in heart and liver. Highly expressed in cell lines derived from nervous system tumors arising from glia, low or absent in non-glial-derived nervous system tumor cell lines. Also found in fetal kidney. According to PubMed:7607567 it is expressed only in brain tumor glioblastoma multiforme/astrocytoma and not in other nervous system tumors or normal fetal or adult tissues.
  • 序列相似性Belongs to the CRISP family.
  • 细胞定位Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CRISP 7 antibody
    • CRISP7 antibody
    • GLI pathogenesis related 1 antibody
    • GLI pathogenesis-related 1 (glioma) antibody
    • Glioma pathogenesis related protein 1 antibody
    • Glioma pathogenesis-related protein 1 antibody
    • GLIP1_HUMAN antibody
    • GliPR 1 antibody
    • GLIPR antibody
    • GLIPR1 antibody
    • Protein RTVP-1 antibody
    • RELATED TO TESTIS SPECIFIC, VESPID, AND PATHOGENESIS PROTEINS 1 antibody
    • RTVP 1 antibody
    • RTVP1 antibody
    • Testes specific vespid and pathogenesis protein 1 antibody
    • testes-specific vespid and pathogenesis protein 1 antibody
    see all

Anti-GLIPR1 antibody 图像

  • Overlay histogram showing HepG2 cells stained with ab118382 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab118382, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
  • Anti-GLIPR1 antibody (ab118382) at 5 µg/ml + HeLa cell lysate at 50 µg

    Predicted band size : 30 kDa
  • Anti-GLIPR1 antibody (ab118382) at 5 µg/ml + recombinant immunogen at 0.2 µg/ml

    Predicted band size : 30 kDa
    Western blot against tagged recombinant immunogen using ab118382 antibody at 5µg/ml dilution. Predicted band size of immunogen is 34.58kDa.

Anti-GLIPR1 antibody (ab118382)参考文献

ab118382 has not yet been referenced specifically in any publications.

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For GLIPR1, the immunogen for ab118382 is 97% homologous with XP_001117594 - the predicted protein sequence for Macaca mulatta. We would predict this antibody to work for monkey samples.

For GAB1 and M...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"