概述

  • 产品名称
    Anti-Glicentin抗体
    参阅全部 Glicentin 一抗
  • 描述
    兔多克隆抗体to Glicentin
  • 经测试应用
    适用于: IHC-Pmore details
    不适用于: WB
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Hamster, Cow, Pig
  • 免疫原

    Synthetic peptide corresponding to Human Glicentin aa 1-100 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab90220)

  • 阳性对照
    • This antibody gave a positive signal in Human Pancreatic Tissue.

应用

Our Abpromise guarantee covers the use of ab68395 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 1 µg/ml.
  • 应用说明
    Is unsuitable for WB.
  • 靶标

    • 功能
      Glucagon plays a key role in glucose metabolism and homeostasis. Regulates blood glucose by increasing gluconeogenesis and decreasing glycolysis. A counterregulatory hormone of insulin, raises plasma glucose levels in response to insulin-induced hypoglycemia. Plays an important role in initiating and maintaining hyperglycemic conditions in diabetes.
      GLP-1 is a potent stimulator of glucose-dependent insulin release. Play important roles on gastric motility and the suppression of plasma glucagon levels. May be involved in the suppression of satiety and stimulation of glucose disposal in peripheral tissues, independent of the actions of insulin. Have growth-promoting activities on intestinal epithelium. May also regulate the hypothalamic pituitary axis (HPA) via effects on LH, TSH, CRH, oxytocin, and vasopressin secretion. Increases islet mass through stimulation of islet neogenesis and pancreatic beta cell proliferaton. Inhibits beta cell apoptosis.
      GLP-2 stimulates intestinal growth and up-regulates villus height in the small intestine, concomitant with increased crypt cell proliferation and decreased enterocyte apoptosis. The gastrointestinal tract, from the stomach to the colon is the principal target for GLP-2 action. Plays a key role in nutrient homeostasis, enhancing nutrient assimilation through enhanced gastrointestinal function, as well as increasing nutrient disposal. Stimulates intestinal glucose transport and decreases mucosal permeability.
      Oxyntomodulin significantly reduces food intake. Inhibits gastric emptying in humans. Suppression of gastric emptying may lead to increased gastric distension, which may contribute to satiety by causing a sensation of fullness.
      Glicentin may modulate gastric acid secretion and the gastro-pyloro-duodenal activity. May play an important role in intestinal mucosal growth in the early period of life.
    • 组织特异性
      Glucagon is secreted in the A cells of the islets of Langerhans. GLP-1, GLP-2, oxyntomodulin and glicentin are secreted from enteroendocrine cells throughout the gastrointestinal tract. GLP1 and GLP2 are also secreted in selected neurons in the brain.
    • 序列相似性
      Belongs to the glucagon family.
    • 翻译后修饰
      Proglucagon is post-translationally processed in a tissue-specific manner in pancreatic A cells and intestinal L cells. In pancreatic A cells, the major bioactive hormone is glucagon cleaved by PCSK2/PC2. In the intestinal L cells PCSK1/PC1 liberates GLP-1, GLP-2, glicentin and oxyntomodulin. GLP-1 is further N-terminally truncated by post-translational processing in the intestinal L cells resulting in GLP-1(7-37) GLP-1-(7-36)amide. The C-terminal amidation is neither important for the metabolism of GLP-1 nor for its effects on the endocrine pancreas.
    • 细胞定位
      Secreted.
    • Information by UniProt
    • 数据库链接
    • 形式
      Cleaved into the following 8 chains: 1.Glicentin; 2.Glicentin-related polypeptide = GRPP; 3.Oxyntomodulin = OXM = OXY; 4.Glucagon; 5.Glucagon-like peptide 1 = GLP-1; 6.Glucagon-like peptide 1(7-37) = GLP-1(7-37); 7.Glucagon-like peptide 1(7-36) = GLP-1(7-36); 8.Glucagon-like peptide 2 = GLP-2.
    • 别名
      • GCG antibody
      • glicentin-related polypeptide antibody
      • GLP-1 antibody
      • GLP-1(7-36) antibody
      • GLP-1(7-37) antibody
      • GLP-2 antibody
      • GLUC_HUMAN antibody
      • Glucagon antibody
      • glucagon-like peptide 1 antibody
      • Glucagon-like peptide 2 antibody
      • GRPP antibody
      • OXM antibody
      • OXY antibody
      see all

    Anti-Glicentin antibody 图像

    • IHC image of ab68395 staining in mouse pancreas formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab68395, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    Anti-Glicentin antibody (ab68395)参考文献

    ab68395 has not yet been referenced specifically in any publications.

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