Glibenclamide (Glyburide) (ab120267)

概述

  • 产品名称Glibenclamide (Glyburide)
  • 描述
    K+ channel blocker
  • 别名
    • Glyburide
  • 生物学描述Selective blocker of ATP-sensitive (KIR6.x) inward rectifier K+ channels.
  • 纯度> 99%

性能

    应用

    Our Abpromise guarantee covers the use of ab120267 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    应用 Ab评论 说明
    Functional Studies Use at an assay dependent concentration.

    Glibenclamide (Glyburide) 图像

    • MEF1 cells were incubated at 37°C for 24h with vehicle control (0 µM) and 5 µM of glibenclamide (ab120267) in DMSO. Increased expression of JNK1+JNK2 (phospho T183 + Y185) (ab4821) correlates with an increase in glibenclamide concentration, as described in literature.

      Whole cell lysates were prepared with RIPA buffer (containing protease inhibitors and sodium orthovanadate), 10 µg of each were loaded on the gel and the WB was run under reducing conditions. After transfer the membrane was blocked for an hour using 3% milk before being incubated with ab4821at 1/1000 dilution and ab85139 at 1 µg/ml overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP (ab97051) at 1/10000 dilution and visualised using ECL development solution.

    Glibenclamide (Glyburide) (ab120267)参考文献

    This product has been referenced in:
    • Legtenberg RJ  et al. The role of myocardial KATP-channel blockade in the protective effects of glibenclamide against ischaemia in the rat heart. Pharmacol Toxicol 91:51-6 (2002). Read more (PubMed: 12420792) »
    • Babenko AP  et al. A view of sur/KIR6.X, KATP channels. Annu Rev Physiol 60:667-87 (1998). Read more (PubMed: 9558481) »
    • Light PE & French RJ Glibenclamide selectively blocks ATP-sensitive K+ channels reconstituted from skeletal muscle. Eur J Pharmacol 259:219-22 (1994). Read more (PubMed: 7982447) »

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