Anti-Gliadin抗体[14D5] (ab36729)

概述

  • 产品名称
    Anti-Gliadin抗体[14D5]
    参阅全部 Gliadin 一抗
  • 描述
    小鼠单克隆抗体[14D5] to Gliadin
  • 特异性
    This antibody reacts specifically with the deamidated peptide (KLQPFPQPELPYPQPQ) when coated directly in an immunoplate. Does not react with Oats. This antibody reacts strongly with the deamidated peptide but cross-reacts about 5% with the non-deamidated peptide.
  • 经测试应用
    适用于: ELISA, WBmore details
  • 种属反应性
    与反应: Triticum aestivum, Hordeum vulgare
  • 免疫原

    Gliadin-related peptide Lys57-Glu65-[alpha-gliadin (58-73)]

    KLQPFPQPELPYPQPQ

    ) coupled to immunogenic carrier protein.

  • 表位
    The epitope is located in the Lys57-Glu65-[alpha-gliadin (58-73)] (KLQPFPQPELPYPQPQ) peptide.
  • 常规说明


    The immunogen corresponds to a deamidated form of a region that includes the T-cell epitopes, including the immunodominant PQPQLPY region and two PXPQP motifs associated with binding to IgA from patients with celiac disease. Complete homology exists between residues 63-73, 70-80 and 77-87 of wheat alpha gliadin.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液
    Preservative: 15mM Sodium Azide
    Constituents: 0.5M Sodium chloride, 0.01M PBS, pH 7.4
  • Concentration information loading...
  • 纯度
    Protein A purified
  • Primary antibody说明
    The immunogen corresponds to a deamidated form of a region that includes the T-cell epitopes, including the immunodominant PQPQLPY region and two PXPQP motifs associated with binding to IgA from patients with celiac disease. Complete homology exists between residues 63-73, 70-80 and 77-87 of wheat alpha gliadin.
  • 克隆
    单克隆
  • 克隆编号
    14D5
  • 骨髓瘤
    x63-Ag8.653
  • 同种型
    IgG2a
  • 轻链类型
    kappa
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab36729 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use at an assay dependent concentration.
WB Use at an assay dependent concentration.

dilution 1/1000

靶标

  • 相关性
    Celiac disease is associated with a CD4+ T-cell response to epitopes of gliadin presented by HLA-DQ2 or -DQ8 class II MHC molecules. These epitopes are present in a 33-mer peptide of wheat alpha-gliadin, residues 56-88, which is resistant to digestion and forms a substrate for tissue transglutaminase (TG2), generating the glutamic acid residues essential for binding to HLA-DQ2. The alcohol soluble proteins (prolamins) from wheat, rye, barley and oats produce the harmful effect of coeliac disease or gluten sensitive enteropathy in humans by causing characteristic changes in the intestinal mucosa. Patients so affected must avoid eating these grains and replace them with rice, corn, potatoes, etc. Many gluten-free foods are produced industrially, thus several immunoassays have been developed for determination of gliadin in supposedly gluten-free foods.
  • 细胞定位
    Cytoplasmic; major seed storage protein in wheat.
  • 别名
    • prolamin antibody

Anti-Gliadin antibody [14D5] 图像

  • All lanes : Anti-Gliadin antibody [14D5] (ab36729) at 1/1000 dilution

    Lane 1 : Rye Lysate
    Lane 2 : Barley Lysate
    Lane 3 : Wheat Lysate


    Performed under reducing conditions.

实验方案

Anti-Gliadin antibody [14D5] (ab36729)参考文献

ab36729 has not yet been referenced specifically in any publications.

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