Anti-Gliadin抗体[14D5] (ab36729)
Key features and details
- Mouse monoclonal [14D5] to Gliadin
- Suitable for: WB
- Reacts with: Wheat, Hordeum vulgare
- Isotype: IgG2a
概述
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产品名称
Anti-Gliadin抗体[14D5] -
描述
小鼠单克隆抗体[14D5] to Gliadin -
宿主
Mouse -
特异性
This antibody reacts specifically with the deamidated peptide (KLQPFPQPELPYPQPQ) when coated directly in an immunoplate. Does not react with Oats. This antibody reacts strongly with the deamidated peptide but cross-reacts about 5% with the non-deamidated peptide. -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Wheat, Hordeum vulgare, Secale cereale -
免疫原
Gliadin-related peptide Lys57-Glu65-[alpha-gliadin (58-73)]
KLQPFPQPELPYPQPQ
) coupled to immunogenic carrier protein. -
表位
The epitope is located in the Lys57-Glu65-[alpha-gliadin (58-73)] (KLQPFPQPELPYPQPQ) peptide. -
常规说明
The immunogen corresponds to a deamidated form of a region that includes the T-cell epitopes, including the immunodominant PQPQLPY region and two PXPQP motifs associated with binding to IgA from patients with celiac disease. Complete homology exists between residues 63-73, 70-80 and 77-87 of wheat alpha gliadin.
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性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.40
Preservative: 0.097% Sodium azide
Constituents: 2.9% Sodium chloride, 0.0268% PBS -
Concentration information loading...
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纯度
Protein A purified -
Primary antibody说明
The immunogen corresponds to a deamidated form of a region that includes the T-cell epitopes, including the immunodominant PQPQLPY region and two PXPQP motifs associated with binding to IgA from patients with celiac disease. Complete homology exists between residues 63-73, 70-80 and 77-87 of wheat alpha gliadin. -
克隆
单克隆 -
克隆编号
14D5 -
骨髓瘤
x63-Ag8.653 -
同种型
IgG2a -
轻链类型
kappa -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab36729于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
1/1000.
dilution 1/1000 |
说明 |
---|
WB
1/1000. dilution 1/1000 |
靶标
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相关性
Celiac disease is associated with a CD4+ T-cell response to epitopes of gliadin presented by HLA-DQ2 or -DQ8 class II MHC molecules. These epitopes are present in a 33-mer peptide of wheat alpha-gliadin, residues 56-88, which is resistant to digestion and forms a substrate for tissue transglutaminase (TG2), generating the glutamic acid residues essential for binding to HLA-DQ2. The alcohol soluble proteins (prolamins) from wheat, rye, barley and oats produce the harmful effect of coeliac disease or gluten sensitive enteropathy in humans by causing characteristic changes in the intestinal mucosa. Patients so affected must avoid eating these grains and replace them with rice, corn, potatoes, etc. Many gluten-free foods are produced industrially, thus several immunoassays have been developed for determination of gliadin in supposedly gluten-free foods. -
细胞定位
Cytoplasmic; major seed storage protein in wheat. -
数据库链接
- Entrez Gene: 543191 Wheat
- SwissProt: P02863 Wheat
- SwissProt: P04721 Wheat
- SwissProt: P04722 Wheat
- SwissProt: P04723 Wheat
- SwissProt: P04724 Wheat
- SwissProt: P04725 Wheat
- SwissProt: P04726 Wheat
see all -
别名
- prolamin antibody
图片
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab36729 被引用在 3 文献中.
- Méndez PX et al. The Ability of the Yeast Wickerhamomyces anomalus to Hydrolyze Immunogenic Wheat Gliadin Proteins. Foods 11:N/A (2022). PubMed: 36553848
- Manai F et al. The In Vitro Effects of Enzymatic Digested Gliadin on the Functionality of the Autophagy Process. Int J Mol Sci 19:N/A (2018). ICC/IF . PubMed: 29473905
- Engstrom N et al. Towards Celiac-safe foods: Decreasing the affinity of transglutaminase 2 for gliadin by addition of ascorbyl palmitate and ZnCl2 as detoxifiers. Sci Rep 7:77 (2017). PubMed: 28250436