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Our Abpromise guarantee covers the use of ab26056 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 4 µg/ml. Predicted molecular weight: 133 kDa.Can be blocked with Gli2 peptide (ab130900).
Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
|ICC/IF||Use at an assay dependent concentration.|
|ICC||1/200. See Abreview|
|EMSA||Use a concentration of 1 µg/ml.|
|ChIP||Use at an assay dependent concentration.|
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"