Anti-GLG1抗体(ab103439)

概述

  • 产品名称Anti-GLG1抗体
    参阅全部 GLG1 一抗
  • 描述
    兔多克隆抗体to GLG1
  • 经测试应用适用于: WB, IHC-P, IHC-FoFr, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Chicken, Human
  • 免疫原

    Synthetic peptide corresponding to Human GLG1 aa 1150-1179 (C terminal) conjugated to keyhole limpet haemocyanin.

  • 阳性对照
    • MCF7 cell line and Mouse cerebellum tissue lysates; skeletal muscle tissue. This antibody gave a positive result when used in the following formaldehyde fixed cell line: HepG2.

性能

应用

Our Abpromise guarantee covers the use of ab103439 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/100 - 1/500. Predicted molecular weight: 135 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IHC-FoFr Use at an assay dependent concentration.
ICC/IF 1/200.

Anti-GLG1 antibody 图像

  • ICC/IF image of ab103439 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab103439 at 1/200 dilution overnight at +4°C. The secondary antibody (green) was DyLight® 488 Goat anti-Rabbit IgG (H+L) (ab96899) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Anti-GLG1 antibody (ab103439) at 1/100 dilution + MCF7 cell line lysates at 35 µg

    Predicted band size : 135 kDa
  • Anti-GLG1 antibody (ab103439) at 1/100 dilution + Mouse cerebellum tissue lysates at 35 µg

    Predicted band size : 135 kDa
  • ab103439 at 1/50 dilution, staining Golgi Complex in Human skeletal muscle by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue, followed by peroxidase-conjugated secondary antibody and DAB staining.
  • ab103439 staining Golgi Complex in murine spinal cord motor neurons by Immunohistochemistry (PFA perfusion fixed frozen sections).
    Tissue was fixed in paraformaldehyde, permeabilized using 0.2% Triton/PBS, blocked with 2.5% serum for 1 hour at 20°C and then incubated with ab103439 at a 1/500 dilution for 12 hours at 4°C. The secondary used was an Alexa-Fluor 488 conjugated goat anti-rabbit polyclonal used at a 1/500 dilution.

    See Abreview

Anti-GLG1 antibody (ab103439)参考文献

ab103439 has not yet been referenced specifically in any publications.

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
Sample Chicken Cell (Primary oculomotor culture)
Specification Primary oculomotor culture
Permeabilization Yes - PBS + 0.1% Tween20
Fixative Formaldehyde
Username

Abcam user community

Verified customer

提交于 Oct 27 2014

Application Western blot
Loading amount 100000 cells
Gel Running Conditions Reduced Denaturing (10%)
Sample Human Cell lysate - whole cell (Breast Cancer MDA-MB-231 Cells/ Melanoma MDA-MB-43)
Specification Breast Cancer MDA-MB-231 Cells/ Melanoma MDA-MB-43
Blocking step Odyssey Blocking Buffer as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 50% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Aug 28 2014

Thank you for taking time to contact us and forward an image of your staining. I am sorry to hear that this antibody is not providing satisfactory results.

The details provided will enable us to investigate this case and will provide us with...

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Merci de nous avoir contactés.

Nous avons maintenantvalidé la commande. Le produit doit être envoyé de notre stock des Etats-Unis, vous devriez le recevoir mercredi 29 février.

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Mouse Tissue sections (Spinal cord motor neuron)
Specification Spinal cord motor neuron
Fixative Paraformaldehyde
Antigen retrieval step None
Permeabilization Yes - PBS Triton 0.1%
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2.5% · Temperature: 20°C
Username

Dr. Georg Haase

Verified customer

提交于 Feb 24 2012

Code de remise : ********
Date d’expiration : JJ MM AAAA

Je suis ravi que vous souhaitiez participerà notre offre et tester ab103439 en cytométrie en flux et/ou IHC-Fr. Le code de réduction correspondant est *...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"