概述

  • 产品名称Anti-GLE1抗体
    参阅全部 GLE1 一抗
  • 描述
    兔多克隆抗体to GLE1
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Horse, Chicken, Cow, Cat, Dog, Pig, Saccharomyces cerevisiae
  • 免疫原

    Synthetic peptide corresponding to a region within N terminal ammino acids 217-266 (LKLREAEQQRVKQAEQERLRKEEGQIRLRALYALQEEMLQLSQQLDASE Q) of human GLE1 (NP_001490).

  • 阳性对照
    • Placenta lysate

性能

应用

Our Abpromise guarantee covers the use of ab81648 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 80 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/1562500.

靶标

  • 功能Required for the export of mRNAs containing poly(A) tails from the nucleus into the cytoplasm. May be involved in the terminal step of the mRNA transport through the nuclear pore complex (NPC).
  • 疾病相关Defects in GLE1 are the cause of lethal congenital contracture syndrome type 1 (LCCS1) [MIM:253310]; also known as multiple contracture syndrome type Finnish. LCCS is an autosomal recessive disorder characterized by early fetal hydrops and akinesia, micrognatia, pulmonary hypoplasia, pterygia, multiple joint contractures, specific neuropathology with degeneration of anterior horn neurons and extreme skeletal muscle atrophy. LCCS1 leads to prenatal death.
    Defects in GLE1 are the cause of lethal arthrogryposis with anterior horn cell disease (LAAHD) [MIM:611890]. LAAHD is characterized by fetal akinesia, arthrogryposis and motor neuron loss. LAADH fetus often survive delivery, but die early as a result of respiratory failure. Neuropathological findings resemble those of LCCS1, but are less severe.
  • 序列相似性Belongs to the GLE1 family.
  • 细胞定位Nucleus. Cytoplasm. Shuttles between the nucleus and the cytoplasm. Shuttling is essential for its mRNA export function and Cytoplasm. Nucleus > nuclear pore complex. Shuttles between the nucleus and the cytoplasm. In the nucleus, isoform 1 localizes to the nuclear pore complex and nuclear envelope. Shuttling is essential for its mRNA export function.
  • Information by UniProt
  • 数据库链接
  • 别名
    • GLE 1 antibody
    • GLE1 antibody
    • GLE1 like protein antibody
    • GLE1 like RNA export mediator antibody
    • GLE1 RNA export mediator homolog antibody
    • GLE1 RNA export mediator like (yeast) antibody
    • GLE1-like protein antibody
    • GLE1_HUMAN antibody
    • GLE1L antibody
    • hGLE1 antibody
    • LCCS 1 antibody
    • LCCS antibody
    • LCCS1 antibody
    • Nucleoporin GLE1 antibody
    see all

Anti-GLE1 antibody 图像

  • Anti-GLE1 antibody (ab81648) at 1 µg/ml + placenta lysate at 10 ug protein.

    Secondary
    HRP conjugated anti-Rabbit IgG diluted 1/50,000 - 1/100,000.

    Predicted band size : 80 kDa
    Observed band size : 70 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 35 kDa. We are unsure as to the identity of these extra bands.

Anti-GLE1 antibody (ab81648)参考文献

ab81648 has not yet been referenced specifically in any publications.

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