• 产品名称Anti-GLCNE抗体
    参阅全部 GLCNE 一抗
  • 描述
    兔多克隆抗体to GLCNE
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Synthetic peptide conjugated to KLH, corresponding to a region within C terminal amino acids 588-618 of Human GLCNE (NP_005467.1).

  • 阳性对照
    • Mouse kidney tissue lysates.


  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C long term.
  • 存储溶液Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明ab103843 is purified through a protein A column, followed by peptide affinity purification.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域


Our Abpromise guarantee covers the use of ab103843 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/100 - 1/500. Predicted molecular weight: 79 kDa.


  • 功能Regulates and initiates biosynthesis of N-acetylneuraminic acid (NeuAc), a precursor of sialic acids. Plays an essential role in early development (By similarity). Required for normal sialylation in hematopoietic cells. Sialylation is implicated in cell adhesion, signal transduction, tumorigenicity and metastatic behavior of malignant cells.
  • 组织特异性Highest expression in liver and placenta. Also found in heart, brain, lung, kidney, skeletal muscle and pancreas. Isoform 1 is expressed in heart, brain, kidney, liver, placenta, lung, spleen, pancreas, skeletal muscle and colon. Isoform 2 is expressed mainly in placenta, but also in brain, kidney, liver, lung, pancreas and colon. Isoform 3 is expressed at low level in kidney, liver, placenta and colon.
  • 通路Amino-sugar metabolism; N-acetylneuraminate biosynthesis.
  • 疾病相关Defects in GNE are a cause of sialuria (SIALURIA) [MIM:269921]; also known as sialuria French type. In sialuria, free sialic acid accumulates in the cytoplasm and gram quantities of neuraminic acid are secreted in the urine. The metabolic defect involves lack of feedback inhibition of UDP-GlcNAc 2-epimerase by CMP-Neu5Ac, resulting in constitutive overproduction of free Neu5Ac. Clinical features include variable degrees of developmental delay, coarse facial features and hepatomegaly. Sialuria inheritance is autosomal dominant.
    Defects in GNE are the cause of inclusion body myopathy type 2 (IBM2) [MIM:600737]. Hereditary inclusion body myopathies are a group of neuromuscular disorders characterized by adult onset, slowly progressive distal and proximal weakness and a typical muscle pathology including rimmed vacuoles and filamentous inclusions. IBM2 is an autosomal recessive disorder affecting mainly leg muscles, but with an unusual distribution that spares the quadriceps as also observed in Nonaka myopathy.
    Defects in GNE are the cause of Nonaka myopathy (NM) [MIM:605820]; also known as distal myopathy with rimmed vacuoles (DMRV). NM is an autosomal recessive muscular disorder, allelic to inclusion body myopathy 2. It is characterized by weakness of the anterior compartment of the lower limbs with onset in early adulthood, and sparing of the quadriceps muscles. As the inclusion body myopathy, NM is histologically characterized by the presence of numerous rimmed vacuoles without inflammatory changes in muscle specimens.
  • 序列相似性In the N-terminal section; belongs to the UDP-N-acetylglucosamine 2-epimerase family.
    In the C-terminal section; belongs to the ROK (NagC/XylR) family.
  • 翻译后修饰Phosphorylated by PKC.
  • 细胞定位Cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 2310066H07Rik antibody
    • Bifunctional UDP N acetylglucosamine 2 epimerase/N acetylmannosamine kinase antibody
    • DMRV antibody
    • GLCNE_HUMAN antibody
    • Glucosamine (UDP N acetyl) 2 epimerase/N acetylmannosamine kinase antibody
    • GNE antibody
    • IBM2 antibody
    • ManAc kinase antibody
    • N acylmannosamine kinase antibody
    • N-acetylmannosamine kinase antibody
    • NM antibody
    • RP23-209M8.6 antibody
    • Uae1 antibody
    • UDP GlcNAc 2 epimerase antibody
    • UDP GlcNAc 2 epimerase/ManAc kinase antibody
    • UDP N acetylglucosamine 2 epimerase/N acetylmannosamine kinase antibody
    • UDP-GlcNAc-2-epimerase antibody
    • UDP-GlcNAc-2-epimerase/ManAc kinase antibody
    • Uridine diphosphate N acetylglucosamine 2 epimerase antibody
    • Uridine diphosphate-N-acetylglucosamine-2-epimerase antibody
    see all

Anti-GLCNE antibody 图像

  • Anti-GLCNE antibody (ab103843) at 1/100 dilution + Mouse kidney tissue lysates at 35 µg

    Predicted band size : 79 kDa

Anti-GLCNE antibody (ab103843)参考文献

ab103843 has not yet been referenced specifically in any publications.

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Thank you for providing that extra information. This will aid in investigating the complaint further and initiating any additional testing which may be required.

Having reviewed the extra protocol details I cannot identify a particular issue...

Read More
Application Western blot
Sample Human Cell lysate - whole cell (colon cancer and breast cancer)
Loading amount 50 µg
Specification colon cancer and breast cancer
Gel Running Conditions Reduced Denaturing (4-20% gel)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 22°C

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提交于 Mar 06 2012