Horse, Guinea pig, Cow, Dog, Chimpanzee, Rhesus monkey, Gorilla, Elephant
Synthetic peptide, corresponding to a region between N terminal amino acids 1-50 of Human GKAP1 (NP_079487.2).
293T, HeLa and Jurkat whole cell lysates.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate Note: pH 7 to 8
Concentration information loading...
Immunogen affinity purified
ab123594 was affinity purified using an epitope specific to GKAP1 immobilized on solid support.
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000 - 1/10000.
Use at 2-10 µg/mg of lysate.
Anti-GKAP1 antibody 图像
Western blot - Anti-GKAP1 antibody (ab123594)
All lanes : Anti-GKAP1 antibody (ab123594) at 0.1 µg/ml Lane 1 : 293T whole cell lysate at 50 µg Lane 2 : 293T whole cell lysate at 15 µg Lane 3 : HeLa whole cell lysate at 50 µg Lane 4 : Jurkat whole cell lysate at 50 µg Developed using the ECL technique Exposure time : 10 seconds
Immunoprecipitation - Anti-GKAP1 antibody (ab123594)
Detection of GKAP1 by Western Blot of Immunprecipitate.
ab123594 at 1µg/ml staining GKAP1 in 293T whole cell lysate immunoprecipitated using ab123594 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 10 seconds.
Anti-GKAP1 antibody (ab123594)参考文献
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"