Mouse, Rat, Cow, Pig
Recombinant protein fragment corresponding to a region within amino acids 117 and 308 of GK2 (NP_149991).
WB: H1299, HeLa or HepG2 cell lysate;
ICC/IF: HeLa cells.
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol, 0.1M Tris, 0.1M Glycine, pH 7
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/3000. Predicted molecular weight: 61 kDa.
1/100 - 1/200.
GK2 (glycerol kinase 2) is a key enzyme in the regulation of glycerol uptake and metabolism. It is a phosphotransferase enzyme involved in lipolysis and catalyzes the transfer of a phosphate from ATP to glycerol thus forming glycerol phosphate. This protein could be the product of a pseudogene.
Mitochondrion outer membrane; Peripheral membrane protein; Cytoplasmic side (By similarity). Cytoplasm (By similarity). In sperm the majority of the enzyme is bound to mitochondria.
ATP:glycerol 3 phosphotransferase antibody
EC 184.108.40.206 antibody
GK 2 antibody
Western blot - Anti-GK2 antibody (ab96818)
Anti-GK2 antibody (ab96818) at 1/1000 dilution + HepG2 whole cell lysate at 30 µg
Predicted band size: 61 kDa 7.5% SDS PAGE
Immunocytochemistry/ Immunofluorescence - Anti-GK2 antibody (ab96818)
1: Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells, using ab96818 at 1/200 dilution.
2: image merged with DNA probe.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"