概述

  • 产品名称
  • 描述
    兔多克隆抗体to GJE1
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    A region within synthetic peptide: YFLTSESTRR HKKATDSLPV VETKEQFQEA VPGRSLAQEK QRPVGPRDA, corresponding to C terminal amino acids 231-279 of Human GJE1

  • 阳性对照
    • Jurkat cell lysate.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 纯度
    Protein A purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab66618 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 2.5 µg/ml. Detects a band of approximately 31 kDa (predicted molecular weight: 31 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 功能
    One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell.
  • 组织特异性
    CNS specific. Expression is restricted to brain, spinal cord, and sciatic nerve. According to PubMed:12881038, expression is abundant in skeletal muscle, liver, and heart, and to a minor degree in pancreas and kidney.
  • 序列相似性
    Belongs to the connexin family. Gamma-type subfamily.
  • 细胞定位
    Cell membrane. Cell junction > gap junction.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Connexin 29 antibody
    • Connexin 30.2 antibody
    • Connexin 31.3 antibody
    • Connexin-30.2 antibody
    • Connexin-31.3 antibody
    • CX23 antibody
    • Cx29 antibody
    • Cx30.2 antibody
    • Cx31.3 antibody
    • CXG3_HUMAN antibody
    • Gap junction epsilon 1 protein antibody
    • Gap junction epsilon-1 protein antibody
    • Gap junction gamma 3 protein antibody
    • Gap junction gamma-3 protein antibody
    • Gap junction protein epsilon 1 29kDa antibody
    • Gap junction protein epsilon 1 antibody
    • Gap junction protein gamma 3 30.2kDa antibody
    • Gap junction protein gamma 3 antibody
    • GJC 3 antibody
    • GJC3 antibody
    • GJE 1 antibody
    see all

Anti-GJE1 antibody 图像

  • Anti-GJE1 antibody (ab66618) at 2.5 µg/ml + Jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti-rabbit IgG at 1/50000 dilution

    Predicted band size : 31 kDa
    Observed band size : 31 kDa
    Additional bands at : 42 kDa,45 kDa. We are unsure as to the identity of these extra bands.

实验方案

Anti-GJE1 antibody (ab66618)参考文献

ab66618 has not yet been referenced specifically in any publications.

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