概述

  • 产品名称Anti-GIRK2抗体
    参阅全部 GIRK2 一抗
  • 描述
    山羊多克隆抗体to GIRK2
  • 经测试应用适用于: IHC-P, WB, ELISA, IHC-FrFl, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
    预测可用于: Syrian Hamster, Orangutan
  • 免疫原

    Synthetic peptide:

    C-SSKLNQHAELET

    , corresponding to C terminal amino acids 386-397 of Human GIRK2

  • 阳性对照
    • Human Brain (Substantia Nigra) lysate.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris saline, pH 7.3
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab65096 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/500 - 1/1000.
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 47 kDa).
ELISA Use at an assay dependent concentration.
IHC-FrFl Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration. PubMed: 24586273

靶标

  • 功能This potassium channel may be involved in the regulation of insulin secretion by glucose and/or neurotransmitters acting through G-protein-coupled receptors. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium.
  • 组织特异性Most abundant in cerebellum, and to a lesser degree in islets and exocrine pancreas.
  • 疾病相关Keppen-Lubinsky syndrome
  • 序列相似性Belongs to the inward rectifier-type potassium channel (TC 1.A.2.1) family. KCNJ6 subfamily.
  • 细胞定位Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • inwardly rectifying subfamily J member 6 antibody
    • BIR1 antibody
    • G protein activated inward rectifier potassium channel 2 antibody
    • G protein-activated inward rectifier potassium channel 2 antibody
    • GIRK-2 antibody
    • hiGIRK2 antibody
    • Inward rectifier K(+) channel Kir3.2 antibody
    • Inward rectifier potassium channel KIR3.2 antibody
    • KATP-2 antibody
    • KATP2 antibody
    • KCNJ6 antibody
    • KCNJ6_HUMAN antibody
    • Kcnj7 antibody
    • KIR3.2 antibody
    • Potassium channel antibody
    • Potassium channel inwardly rectifying subfamily J member 6 antibody
    • Potassium inwardly rectifying channel subfamily J member 6 antibody
    see all

Anti-GIRK2 antibody 图像

  • Ab65096 staining of GIRK2 in rat brain (substantia nigra) tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Heat-mediated antigen retrieval was carried out using citric acid. Samples were incubated with primary antibody (1/1000) for two hours at room temperature. A Biotin-conjugated horse anti-goat IgG polyclonal was used as the secondary antibody.

  • Ab65096 staining of GIRK2 in mouse brain (hippocampus) tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Heat-mediated antigen retrieval was carried out using citric acid. Samples were incubated with primary antibody (1/500) for two hours at room temperature. A Biotin-conjugated horse anti-goat IgG polyclonal was used as the secondary antibody.

  • Ab65096 staining of GIRK2 in mouse brain (substantia nigra) tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Heat-mediated antigen retrieval was carried out using citric acid. Samples were incubated with primary antibody (1/500) for two hours at room temperature. A Biotin-conjugated horse anti-goat IgG polyclonal was used as the secondary antibody.

  • Anti-GIRK2 antibody (ab65096) at 1 µg/ml + Human Brain (Substantia Nigra) lysate (35µg protein in RIPA buffer).

    Predicted band size : 47 kDa
    Observed band size : 48 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 35 kDa. We are unsure as to the identity of these extra bands.
    Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-GIRK2 antibody (ab65096)参考文献

This product has been referenced in:
  • Hartfield EM  et al. Physiological characterisation of human iPS-derived dopaminergic neurons. PLoS One 9:e87388 (2014). ICC/IF ; Human . Read more (PubMed: 24586273) »

See 1 Publication for this product

Product Wall

Application Immunohistochemistry free floating
Sample Meriones unguiculatus Tissue sections (Brain)
Specification Brain
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提交于 Oct 31 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"