概述

  • 产品名称Anti-Girdin抗体
    参阅全部 Girdin 一抗
  • 描述
    兔多克隆抗体to Girdin
  • 经测试应用适用于: WB, ELISA, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide, derived from Human Girdin, conjugated to KLH at the C terminal

  • 阳性对照
    • Human tonsil tissue, Rat brain tissue lysate. IF/ICC: Malme-3M cell line.

性能

应用

Our Abpromise guarantee covers the use of ab113890 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 216 kDa.
ELISA Use at an assay dependent concentration.
IHC-P Use a concentration of 5 µg/ml.
ICC/IF Use a concentration of 5 µg/ml.

靶标

  • 功能Plays a role as a key modulator of the AKT-mTOR signaling pathway controlling the tempo of the process of newborn neurons integration during adult neurogenesis, including correct neuron positioning, dendritic development and synapse formation. Enhances phosphoinositide 3-kinase (PI3K)-dependent phosphorylation and kinase activity of AKT1/PKB, but does not possess kinase activity itself. Phosphorylation of AKT1/PKB thereby induces the phosphorylation of downstream effectors GSK3 and FOXO1/FKHR, and regulates DNA replication and cell proliferation (By similarity). Essential for the integrity of the actin cytoskeleton and for cell migration. Required for formation of actin stress fibers and lamellipodia. May be involved in membrane sorting in the early endosome.
  • 组织特异性Expressed ubiquitously.
  • 序列相似性Belongs to the CCDC88 family.
  • 翻译后修饰Phosphorylation is induced by epidermal growth factor (EGF) in a phosphoinositide 3-kinase (PI3K)-dependent manner. Phosphorylation by AKT1/PKB is necessary for the delocalization from the cell membrane and for cell migration.
  • 细胞定位Membrane. Cell membrane. Cytoplasm > cytosol. Cytoplasmic vesicle. Cell projection > lamellipodium. Localizes to the cell membrane through interaction with phosphoinositides.
  • Information by UniProt
  • 数据库链接
  • 别名
    • AKT iphosphorylation enhancer antibody
    • Akt phosphorylation enhancer antibody
    • APE antibody
    • Ccdc88a antibody
    • Coiled coil domain containing 88A antibody
    • Coiled coil domain containing protein 88A antibody
    • Coiled-coil domain-containing protein 88A antibody
    • FLJ10392 antibody
    • G alpha interacting vesicle associated protein antibody
    • G alpha-interacting vesicle-associated protein antibody
    • Galpha interacting vesicle associated protein antibody
    • Girders of actin filament antibody
    • Girdin antibody
    • GIV antibody
    • GRDN antibody
    • GRDN_HUMAN antibody
    • HkRP1 antibody
    • Hook related protein 1 antibody
    • Hook-related protein 1 antibody
    • KIAA1212 antibody
    see all

Anti-Girdin antibody 图像

  • ab113890, at 5 µg/ml, staining Girdin in Formalin-Fixed, Paraffin-Embedded Human tonsil tissue by immunohistochemistry.
  • Lane 1 : Anti-Girdin antibody (ab113890) at 1 µg/ml
    Lane 2 : Anti-Girdin antibody (ab113890) at 2 µg/ml

    Lane 1 : Rat brain lysate
    Lane 2 : Rat brain lysate


    Predicted band size : 216 kDa
  • ICC/IF image of ab113890 stained Malme-3M cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab113890, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-Girdin antibody (ab113890)参考文献

ab113890 has not yet been referenced specifically in any publications.

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