Anti-GilZ / TilZ抗体[3A5] (ab117085)


  • 产品名称Anti-GilZ / TilZ抗体[3A5]
    参阅全部 GilZ / TilZ 一抗
  • 描述
    小鼠单克隆抗体[3A5] to GilZ / TilZ
  • 经测试应用适用于: WB, IHC-P, ICC/IF, Sandwich ELISAmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant fragment, corresponding to amino acids 1-98 of Human GilZ/ TilZ with a proprietary tag (NP_932174).

  • 阳性对照
    • Human breast and tonsil tissues; Transfected 293T lysate; HeLa cells.



Our Abpromise guarantee covers the use of ab117085 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Predicted molecular weight: 15 kDa.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 10 µg/ml.
Sandwich ELISA Use at an assay dependent concentration.


  • 功能Protects T-cells from IL2 deprivation-induced apoptosis through the inhibition of FOXO3A transcriptional activity that leads to the down-regulation of the pro-apoptotic factor BCL2L11. In macrophages, plays a role in the anti-inflammatory and immunosuppressive effects of glucocorticoids and IL10. In T-cells, inhibits anti-CD3-induced NFKB1 nuclear translocation. In vitro, suppresses AP1 and NFKB1 DNA-binding activities.
  • 组织特异性Expressed in brain, lung, spleen and skeletal muscle. Lower levels detected in heart and kidney. Not detected in the pancreas. In non-lymphoid tissues, in the absence of inflammation, the major source of constitutive expression is the macrophage lineage. Also expressed in cells from different hemopoietic cell lineages, including bone marrow cells, CD34+ stem cells, mature B- and T-cells, monocytes and granulocytes. Down-regulated in activated macrophages from inflammatory lesions of delayed-type hypersensitivity (DTH) reactions, such as in tuberculosis and in Crohn disease, whereas in Burkitt lymphoma, persists in macrophages involved in the phagocytosis of apoptotic malignant cells.
  • 序列相似性Belongs to the TSC-22/Dip/Bun family.
  • 结构域The leucine-zipper is involved in homodimerization.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Delta sleep-inducing peptide immunoreactor antibody
    • DIP antibody
    • DSIP immunoreactive leucine zipper protein antibody
    • DSIP-immunoreactive peptide antibody
    • Dsip1 antibody
    • Dsipi antibody
    • GILZ antibody
    • Glucocorticoid-induced leucine zipper protein antibody
    • hDIP antibody
    • Protein DIP antibody
    • T22D3_HUMAN antibody
    • TSC-22-like protein antibody
    • TSC-22-related protein antibody
    • TSC-22R antibody
    • TSC22 domain family protein 3 antibody
    • TSC22 domain family, member 3 antibody
    • TSC22 related inducible leucine zipper 3 antibody
    • TSC22D3 antibody
    see all

Anti-GilZ / TilZ antibody [3A5] 图像

  • ab117085, at 5 µg/ml, staining GilZ / TilZ in Formalin-fixed, Paraffin-embedded Human Breast tissue by Immunohistochemistry.
  • ab117085, at 5 µg/ml, staining GilZ / TilZ in Formalin-fixed, Paraffin-embedded Human Tonsil tissue by Immunohistochemistry.
  • All lanes : Anti-GilZ / TilZ antibody [3A5] (ab117085)

    Lane 1 : 293T cell lysate, transfected with GilZ / TilZ
    Lane 2 : 293T cell lysate, non transfected

    Predicted band size : 15 kDa
  • ab117085 at 10µg/ml dilution staining GilZ / TilZ in HeLa cells by Immunofluorescence.
  • Detection limit for recombinant tagged ab117085 is approximately 0.03 ng/ml as a capture antibody.

Anti-GilZ / TilZ antibody [3A5] (ab117085)参考文献

ab117085 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Burkitt's lymphoma cell line xenograft)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: NaCitrate pH 6
Permeabilization No
Specification Burkitt's lymphoma cell line xenograft
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 15% · Temperature: 20°C
Fixative Formaldehyde

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提交于 Dec 21 2015

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