ab27041 staining Gigaxonin in Human gall bladder. The paraffin embedded tissue was incubated with ab27041 (1/175 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6. Cytoplasmic staining can be clearly seen. Ab27041 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines.