Anti-Giantin抗体[9B6] - Golgi Marker (ab37266)

概述

  • 产品名称
    Anti-Giantin抗体[9B6] - Golgi Marker
    参阅全部 Giantin 一抗
  • 描述
    小鼠单克隆抗体[9B6] to Giantin - Golgi Marker
  • 经测试应用
    适用于: IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Rat, Human
  • 免疫原

    Full length protein of Rat Giantin.

  • 阳性对照
    • HeLa cells.
  • 常规说明

    This antibody clone is manufactured by Abcam.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液
    Preservative: 0.02% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 纯度
    IgG fraction
  • 克隆
    单克隆
  • 克隆编号
    9B6
  • 同种型
    IgG1
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab37266 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/200. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF Use a concentration of 5 - 10 µg/ml.

靶标

  • 功能
    May participate in forming intercisternal cross-bridges of the Golgi complex.
  • 细胞定位
    Golgi apparatus membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 372 kDa Golgi complex associated protein antibody
    • 372 kDa Golgi complex-associated protein antibody
    • GCP antibody
    • GCP372 antibody
    • Giantin antibody
    • GOGB1_HUMAN antibody
    • GOLGB1 antibody
    • Golgi autoantigen golgin subfamily a member 1 antibody
    • Golgi autoantigen golgin subfamily b macrogolgin (with transmembrane signal) 1 antibody
    • Golgi autoantigen, golgin subfamily b, macrogolgin 1 antibody
    • Golgi complex associated protein 372kDa antibody
    • Golgi complex-associated protein 372kDa antibody
    • Golgin B1 antibody
    • Golgin B1, golgi integral membrane protein antibody
    • Golgin subfamily B member 1 antibody
    • GOLIM1 antibody
    • Macrogolgin antibody
    • MGC33154 antibody
    see all

图片

  •  The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab6046, 1µg/ml) and (ab37266, 1µg/ml) overnight at +4°C. The secondary antibodies were ab150115 Alexa Fluor® 647 goat anti-mouse IgG (H+L) used at 2µg/ml for 1h and ab175652 Alexa Fluor® 405 goat anti-rabbit IgG (H+L) used at 2µg/ml for 1h. Nuclear Green LCS1 (ab138904) was used to stain the cell nuclei (green) at a dilution of 1/500.

     

  • ICC/IF image of ab37266 stained human HeLa cells. The cells were methanol fixed (5 min) and incubated with the antibody (ab37266, 1µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal Enhancer was used as the primary blocking agent, 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 WGA was used to label plasma membranes (red).

  • ab37266 (1/200) staining Giantin in assynchronous HeLa cells (green). Cells were fixed in Methanol and counterstained with DAPI (red) in order to highlight the nucleus. Please refer to Abreview for further experimental details.

    See Abreview

  • ICC/IF image of ab37266 stained Hek293 cells. The cells were 4% paraformaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab37266, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h.DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

文献

This product has been referenced in:
  • Starheim KK  et al. Depletion of the human N-terminal acetyltransferase hNaa30 disrupts Golgi integrity and ARFRP1 localization. Biosci Rep 37:N/A (2017). ICC/IF ; Human . Read more (PubMed: 28356483) »
  • Zollo A  et al. Sortilin-Related Receptor Expression in Human Neural Stem Cells Derived from Alzheimer's Disease Patients Carrying the APOE Epsilon 4 Allele. Neural Plast 2017:1892612 (2017). ICC/IF ; Human . Read more (PubMed: 28634550) »

See all 24 Publications for this product

客户评价及客户问答

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Muscle cell line (C2C12))
Permeabilization
Yes - Triton in PBS
Specification
Muscle cell line (C2C12)
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1000µg/mL · Temperature: 21°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Mar 01 2016

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Sample
Human Cell (A549)
Specification
A549
Permeabilization
No
Fixative
Formaldehyde
Username

Dr. Armen Petrosyan

Verified customer

提交于 Oct 29 2014

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Sample
Rat Cell (Rat hepatocytes)
Specification
Rat hepatocytes
Permeabilization
No
Fixative
Formaldehyde
Username

Dr. Armen Petrosyan

Verified customer

提交于 Oct 28 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Caco-2 cell)
Specification
Caco-2 cell
Fixative
Methanol
Permeabilization
No
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

提交于 Mar 27 2013

Thank you for your reply.

For paraffin-embedded tissue sections (IHC-P), we recommend Human Tissue sections (renal cancer (Type II papillary)).

http://www.abcam.com/Giantin-antibody-9B6-Golgi-Marker-ab37266-reviews.html?intabreviewi...

Read More

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.
I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has c...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (human cervic cancer)
Specification
human cervic cancer
Fixative
Methanol
Permeabilization
Yes - 0.1% triton X-100
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Oct 04 2012

I am very pleased to hear you would like to accept our offer and test ab37266 in mouse. This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for mouse and include this code in the “...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (hTERT-RPE1)
Specification
hTERT-RPE1
Fixative
Paraformaldehyde
Permeabilization
Yes - saponin
Blocking step
BSA as blocking agent for 15 minute(s) · Concentration: 0.2% · Temperature: RT°C
Username

Abcam user community

Verified customer

提交于 Mar 07 2011

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa, U2OS, 293T)
Loading amount
30 µg
Specification
HeLa, U2OS, 293T
Gel Running Conditions
Reduced Denaturing (5% SDS-PAGE)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Mr. Antibody Solutions

Verified customer

提交于 Jul 30 2008

1-10 of 12 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

注册