Anti-GHRHR抗体(ab76263)
Key features and details
- Rabbit polyclonal to GHRHR
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
概述
-
产品名称
Anti-GHRHR抗体
参阅全部 GHRHR 一抗 -
描述
兔多克隆抗体to GHRHR -
宿主
Rabbit -
经测试应用
适用于: WB, ICC/IFmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide corresponding to Human GHRHR (C terminal).
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS -
Concentration information loading...
-
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Conjugation kits
-
Isotype control
-
Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab76263于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
1/500 - 1/1000. Predicted molecular weight: 47 kDa. Use BSA for blocking
|
|
ICC/IF |
1/500 - 1/1000.
|
说明 |
---|
WB
1/500 - 1/1000. Predicted molecular weight: 47 kDa. Use BSA for blocking |
ICC/IF
1/500 - 1/1000. |
靶标
-
功能
Receptor for GRF, coupled to G proteins which activate adenylyl cyclase. Stimulates somatotroph cell growth, growth hormone gene transcription and growth hormone secretion. -
组织特异性
Pituitary gland. -
疾病相关
Defects in GHRHR are a cause of growth hormone deficiency isolated type 1B (IGHD1B) [MIM:612781]; also known as dwarfism of Sindh. IGHD1B is an autosomal recessive deficiency of GH which causes short stature. IGHD1B patients have low but detectable levels of GH. -
序列相似性
Belongs to the G-protein coupled receptor 2 family. -
细胞定位
Cell membrane. - Information by UniProt
-
数据库链接
- Entrez Gene: 2692 Human
- Omim: 139191 Human
- SwissProt: Q02643 Human
- Unigene: 767 Human
-
别名
- GHRFR antibody
- GHRH R antibody
- GHRH receptor antibody
see all
图片
-
All lanes : Anti-GHRHR antibody (ab76263) at 1/500 dilution
Lane 1 : Extracts from LOVO cells
Lane 2 : Extracts from LOVO cells with immunizing peptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size: 47 kDa
Observed band size: 47 kDa
Additional bands at: >117 kDa. We are unsure as to the identity of these extra bands. -
Immunofluorescence analysis of HUVEC cells using ab76263 at a 1/500 dilution.
Left image un-treated.
Right image treated with immunizing peptide. -
Immunofluorescence analysis of mouse pituitary cells, staining GHRHR with ab76263.
Cells were fixed with 4% paraformaldehyde and blocked using 4% NGS in PBS with 0.3% Triton X-100 for 1 hour at room temperature. Cells were incubated with primary antibody (1/50) in 4% NGS for 18 hours at 4°C. Slides were washed three times for 15 min with PBS and then incubated in 4% NGS with an AlexaFluor®488-conjugated anti- rabbit secondary antibody (1/1000) for 1 hour at room temperature.
实验方案
数据表及文件
-
SDS download
-
Datasheet download
文献 (19)
ab76263 被引用在 19 文献中.
- Gesmundo I et al. Antagonists of Growth Hormone-Releasing Hormone Inhibit the Growth of Pituitary Adenoma Cells by Hampering Oncogenic Pathways and Promoting Apoptotic Signaling. Cancers (Basel) 13:N/A (2021). PubMed: 34439107
- Cong Z et al. Constitutive signal bias mediated by the human GHRHR splice variant 1. Proc Natl Acad Sci U S A 118:N/A (2021). PubMed: 34599099
- Recinella L et al. Antinflammatory, antioxidant, and behavioral effects induced by administration of growth hormone-releasing hormone analogs in mice. Sci Rep 10:732 (2020). PubMed: 31959947
- Li Z et al. Growth hormone-releasing hormone promotes therapeutic effects of peripheral blood endothelial progenitor cells in ischemic repair. J Endocrinol Invest 43:315-328 (2020). PubMed: 31506908
- Ogawa S et al. Abundant expression of the membrane-anchored protease-regulator RECK in the anterior pituitary gland and its implication in the growth hormone/insulin-like growth factor 1 axis in mice. Mol Cell Endocrinol 508:110790 (2020). PubMed: 32165171