概述

  • 产品名称
  • 描述
    兔多克隆抗体to GHRHR
  • 特异性
    ab28692 recognises GHRH-R.
  • 经测试应用
    适用于: WB, ICC, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide:

    RTRAKWTTPSRSAAKVLTSM

    conjugated to KLH, corresponding to amino acids 403-422 of Human GHRHR

  • 阳性对照
    • human ovarian carcinoma

性能

应用

Our Abpromise guarantee covers the use of ab28692 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/3000. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa).
ICC 1/3000.
IHC-P 1/1000. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.

Perform heat mediated antigen retrieval via the microwave method for 20 minutes in citric acid at pH 6.0.

靶标

  • 功能
    Receptor for GRF, coupled to G proteins which activate adenylyl cyclase. Stimulates somatotroph cell growth, growth hormone gene transcription and growth hormone secretion.
  • 组织特异性
    Pituitary gland.
  • 疾病相关
    Defects in GHRHR are a cause of growth hormone deficiency isolated type 1B (IGHD1B) [MIM:612781]; also known as dwarfism of Sindh. IGHD1B is an autosomal recessive deficiency of GH which causes short stature. IGHD1B patients have low but detectable levels of GH.
  • 序列相似性
    Belongs to the G-protein coupled receptor 2 family.
  • 细胞定位
    Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • GHRFR antibody
    • GHRH R antibody
    • GHRH receptor antibody
    • Ghrhr antibody
    • GHRHR_HUMAN antibody
    • GHRHRpsv antibody
    • GRF R antibody
    • GRF receptor antibody
    • GRFR antibody
    • Growth hormone releasing factor receptor antibody
    • Growth hormone releasing hormone receptor antibody
    • Growth hormone-releasing factor receptor antibody
    • Growth hormone-releasing hormone receptor antibody
    see all

图片

  • ab28692 staining Human GHRHR in human ovarian carcinoma samples by immunohistochemistry using paraffin embedded tissue.

  • Anti-GHRHR antibody (ab28692) at 1/500 dilution + LOVO (Human colon adenocarcinoma cell line) Whole Cell Lysate at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 47 kDa
    Observed band size : 47 kDa
    Additional bands at : 70 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 30 seconds
  • ICC/IF image of ab28692 stained SKNSH cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab28692, 1/200 dilution) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

文献

This product has been referenced in:
  • Bagno LL  et al. Growth hormone-releasing hormone agonists reduce myocardial infarct scar in swine with subacute ischemic cardiomyopathy. J Am Heart Assoc 4: (2015). IHC ; Pig . Read more (PubMed: 25827134) »
  • Jaszberenyi M  et al. Potentiation of cytotoxic chemotherapy by growth hormone-releasing hormone agonists. Proc Natl Acad Sci U S A 111:781-6 (2014). WB . Read more (PubMed: 24379381) »

See all 11 Publications for this product

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