概述

  • 产品名称Anti-GHRH抗体
    参阅全部 GHRH 一抗
  • 描述
    兔多克隆抗体to GHRH
  • 经测试应用适用于: ICC/IF, IHC-Fr, RIAmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide corresponding to Human GHRH aa 1-40.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Concentration information loading...
  • 纯度IgG fraction
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab8899 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/1000.
IHC-Fr 1/200 - 1/800. (See Bloch et al 1986 reference).
RIA Use at an assay dependent concentration. (See Bloch et al 1986 reference).

靶标

  • 功能GRF is released by the hypothalamus and acts on the adenohypophyse to stimulate the secretion of growth hormone.
  • 序列相似性Belongs to the glucagon family.
  • 细胞定位Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • GHRF antibody
    • GHRH antibody
    • GRF antibody
    • Growth hormone releasing factor antibody
    • Growth Hormone Releasing Hormone antibody
    • Growth hormone-releasing factor antibody
    • Growth hormone-releasing hormone antibody
    • INN antibody
    • MGC119781 antibody
    • OTTHUMP00000030915 antibody
    • Sermorelin antibody
    • SLIB_HUMAN antibody
    • Somatocrinin antibody
    • Somatoliberin antibody
    • Somatorelin antibody
    see all

Anti-GHRH antibody 图像

  • ICC/IF image of ab8899 stained MCF7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab8899, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-GHRH antibody (ab8899)参考文献

This product has been referenced in:
  • Rick FG  et al. Antagonists of growth hormone-releasing hormone inhibit growth of androgen-independent prostate cancer through inactivation of ERK and Akt kinases. Proc Natl Acad Sci U S A 109:1655-60 (2012). WB ; Human . Read more (PubMed: 22307626) »

See 1 Publication for this product

Product Wall

The molecular weight of the antibody is approximately 160 kDa. It consists of two heavy chains, which are about 53 kDa, and two light chains, which are 25 kDa.

The protein the antibody recognizes, GHRH, is much smaller. The precursor is 12.4...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"