概述

性能

应用

Our Abpromise guarantee covers the use of ab57222 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 13 kDa.
IHC-P Use a concentration of 3 µg/ml.
ICC/IF Use a concentration of 5 µg/ml.

靶标

  • 功能Ghrelin is the ligand for growth hormone secretagogue receptor type 1 (GHSR). Induces the release of growth hormone from the pituitary. Has an appetite-stimulating effect, induces adiposity and stimulates gastric acid secretion. Involved in growth regulation.
    Obestatin may be the ligand for GPR39. May have an appetite-reducing effect resulting in decreased food intake. May reduce gastric emptying activity and jejunal motility.
  • 组织特异性Highest level in stomach. All forms are found in serum as well. Other tissues compensate for the loss of ghrelin synthesis in the stomach following gastrectomy.
  • 序列相似性Belongs to the motilin family.
  • 翻译后修饰O-octanoylation or O-decanoylation is essential for ghrelin activity. The O-decanoylated forms Ghrelin-27-C10 and Ghrelin-28-C10 differ in the length of the carbon backbone of the carboxylic acid bound to Ser-26. A small fraction of ghrelin, ghrelin-28-C10:1, may be modified with a singly unsaturated carboxylic acid.
    Amidation of Leu-98 is essential for obestatin activity.
  • 细胞定位Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Appetite regulating hormone antibody
    • Ghrelin 27 antibody
    • Ghrelin 28 antibody
    • Ghrelin and obestatin prepropeptide antibody
    • Ghrelin antibody
    • Ghrelin/obestatin prepropeptide antibody
    • ghrl antibody
    • GHRL_HUMAN antibody
    • Growth hormone releasing peptide antibody
    • Growth hormone secretagogue antibody
    • Growth hormone-releasing peptide antibody
    • M46 protein antibody
    • Motilin related peptide antibody
    • Motilin-related peptide antibody
    • MTLRP antibody
    • Obestatin antibody
    • Protein M46 antibody
    see all

Anti-Ghrelin antibody 图像

  • Ghrelin antibody (ab57222) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human stomach.


  • Predicted band size : 13 kDa
    Observed band size : 11 kDa (why is the actual band size different from the predicted?)


    Ghrelin monoclonal antibody (ab57222) used in Western Blot analysis of Ghrelin expression in HeLa cell lysate.
  • Anti-Ghrelin antibody (ab57222) at 1 µg/ml + Recombinant Human Ghrelin protein (ab89233) at 0.1 µg

    Secondary
    Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 2 minutes
  • ICC/IF image of ab57222 stained HeLa cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab57222, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879 Dylight 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-Ghrelin antibody (ab57222)参考文献

This product has been referenced in:

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HK2 cell (human kidney proximal tubule))
Loading amount 30 µg
Specification HK2 cell (human kidney proximal tubule)
Gel Running Conditions Reduced Denaturing (14%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 7% · Temperature: 20°C
Username

Mr. Dongil Kim

Verified customer

提交于 May 03 2013

Regarding your enquiry, I have attached and image and the protocol used to this e-mail

The image depicts thestaining of the original batch offormalin-fixed paraffin-embedded human stomach. The concentration of primary antibody we used is 3 ug...

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Thank you for taking time to complete our questionnaire and for contacting us. I am sorry to hear this antibody is not providing satisfactory results.

The details provided will enable us to investigate this case and will provide us with vita...

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Thank you for your enquiry. According to the originator of ab57222, the immunogen is the full length recombinant human Ghrelin (1 a.a. ~ 118 a.a) protein with GST tag. Unfortunately, we do not have information for the epitope of ab57222. I hope ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"