Anti-Ghrelin抗体(ab104307)

概述

  • 产品名称Anti-Ghrelin抗体
    参阅全部 Ghrelin 一抗
  • 描述
    山羊多克隆抗体to Ghrelin
  • 经测试应用适用于: ELISA, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Pig, Rhesus monkey
  • 免疫原

    Synthetic peptide:

    C-QDILWEEAKEAPADK

    , corresponding to C terminal amino acids 103-117 of Human Ghrelin

  • 阳性对照
    • Human Colon and Human Placenta tissue.

性能

应用

Our Abpromise guarantee covers the use of ab104307 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/8000.
IHC-P Use a concentration of 2 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 功能Ghrelin is the ligand for growth hormone secretagogue receptor type 1 (GHSR). Induces the release of growth hormone from the pituitary. Has an appetite-stimulating effect, induces adiposity and stimulates gastric acid secretion. Involved in growth regulation.
    Obestatin may be the ligand for GPR39. May have an appetite-reducing effect resulting in decreased food intake. May reduce gastric emptying activity and jejunal motility.
  • 组织特异性Highest level in stomach. All forms are found in serum as well. Other tissues compensate for the loss of ghrelin synthesis in the stomach following gastrectomy.
  • 序列相似性Belongs to the motilin family.
  • 翻译后修饰O-octanoylation or O-decanoylation is essential for ghrelin activity. The O-decanoylated forms Ghrelin-27-C10 and Ghrelin-28-C10 differ in the length of the carbon backbone of the carboxylic acid bound to Ser-26. A small fraction of ghrelin, ghrelin-28-C10:1, may be modified with a singly unsaturated carboxylic acid.
    Amidation of Leu-98 is essential for obestatin activity.
  • 细胞定位Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Appetite regulating hormone antibody
    • Ghrelin 27 antibody
    • Ghrelin 28 antibody
    • Ghrelin and obestatin prepropeptide antibody
    • Ghrelin antibody
    • Ghrelin/obestatin prepropeptide antibody
    • ghrl antibody
    • GHRL_HUMAN antibody
    • Growth hormone releasing peptide antibody
    • Growth hormone secretagogue antibody
    • Growth hormone-releasing peptide antibody
    • M46 protein antibody
    • Motilin related peptide antibody
    • Motilin-related peptide antibody
    • MTLRP antibody
    • Obestatin antibody
    • Protein M46 antibody
    see all

Anti-Ghrelin antibody 图像

  • Formalin-fixed, paraffin-embedded Human Colon stained with ab104307 at 2 ug/ml followed by biotinylated anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
  • Formalin-fixed, paraffin-embedded Human Placenta stained with ab104307 at 2 ug/ml followed by biotinylated anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

Anti-Ghrelin antibody (ab104307)参考文献

ab104307 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Horse Tissue sections (gastric glandular mucosa)
Antigen retrieval step None
Permeabilization Yes - triton-x 0.3%
Specification gastric glandular mucosa
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 May 13 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"