概述

  • 产品名称Anti-GGT1抗体[EPR5288]
    参阅全部 GGT1 一抗
  • 描述
    兔单克隆抗体[EPR5288] to GGT1
  • 经测试应用适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human GGT1 aa 1-100. The exact sequence is proprietary.

  • 阳性对照
    • WB: Human fetal liver and human fetal kidney tissue lysates and HepG2 and A549 cell lysates. IHC-P: Human kidney and stomach tissues.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

性能

应用

Our Abpromise guarantee covers the use of ab109427 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Predicted molecular weight: 61 kDa.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See protocols (link: http://www.abcam.com/protocols/ihc-antigen-retrieval-protocol).

靶标

  • 功能Initiates extracellular glutathione (GSH) breakdown, provides cells with a local cysteine supply and contributes to maintain intracelular GSH level. It is part of the cell antioxidant defense mechanism. Catalyzes the transfer of the glutamyl moiety of glutathione to amino acids and dipeptide acceptors. Alternatively, glutathione can be hydrolyzed to give Cys-Gly and gamma glutamate. Isoform 3 seems to be inactive.
  • 组织特异性Detected in fetal and adult kidney and liver, adult pancreas, stomach, intestine, placenta and lung. Isoform 3 is lung-specific. There are several other tissue-specific forms that arise from alternative promoter usage but that produce the same protein.
  • 通路Sulfur metabolism; glutathione metabolism.
  • 疾病相关Defects in GGT1 are a cause of glutathionuria (GLUTH) [MIM:231950]; also known as gamma-glutamyltranspeptidase deficiency. It is an autosomal recessive disease.
  • 序列相似性Belongs to the gamma-glutamyltransferase family.
  • 翻译后修饰N-glycosylated on both chains. Contains hexoses, hexosamines and sialic acid residues. Glycosylation profiles tested in kidney and liver tissues reveal the presence of tissue-specific and site-specific glycan composition, despite the overlap in composition among the N-glycans. A total of 36 glycan compositions, with 40 unique structures are observed. Up to 15 different glycans are observed at a single site, with site-specific variation in glycan composition. The difference in glycosylation profiles in the 2 tissues do not affect the enzyme activity.
  • 细胞定位Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CD224 antibody
    • D22S672 antibody
    • D22S732 antibody
    • Gamma glutamyl transpeptidase antibody
    • Gamma glutamyltransferase 1 antibody
    • Gamma glutamyltranspeptidase 1 antibody
    • Gamma-glutamyltransferase 1 antibody
    • Gamma-glutamyltranspeptidase 1 light chain antibody
    • GGT 1 antibody
    • GGT antibody
    • GGT1 antibody
    • GGT1_HUMAN antibody
    • Glutamyl transpeptidase antibody
    • Glutathione hydrolase 1 antibody
    • GTG antibody
    • Leukotriene C4 hydrolase antibody
    • MGC96892 antibody
    • MGC96904 antibody
    • MGC96963 antibody
    • OTTHUMP00000028921 antibody
    • OTTHUMP00000197959 antibody
    see all

Anti-GGT1 antibody [EPR5288] 图像

  • All lanes : Anti-GGT1 antibody [EPR5288] (ab109427) at 1/1000 dilution (purified)

    Lane 1 : Human fetal liver tissue lysate
    Lane 2 : HepG2 whole cell lysate
    Lane 3 : Human fetal kidney tissue lysate
    Lane 4 : A549 whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size : 61 kDa
    Observed band size : 50-60 kDa (why is the actual band size different from the predicted?)
    Blocking and dilution buffer: 5% NFDM/TBST
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human stomach tissue labelling GGT1 with purified ab109427 at a dilution of 1/100. Heat mediated antigen retrieval was performed using EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

  • All lanes : Anti-GGT1 antibody [EPR5288] (ab109427) at 1/1000 dilution (unpurified)

    Lane 1 : Human fetal liver lysate
    Lane 2 : HepG2 cell lysate
    Lane 3 : Human fetal kidney lysate
    Lane 4 : A549 cell lysate

    Lysates/proteins at 10 µg per lane.


    Predicted band size : 61 kDa
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling GGT1 with unpurified ab109427 at a dilution of 1/100.

Anti-GGT1 antibody [EPR5288] (ab109427)参考文献

This product has been referenced in:
  • Mitsunaga M  et al. Fluorescence endoscopic detection of murine colitis-associated colon cancer by topically applied enzymatically rapid-activatable probe. Gut : (2012). Read more (PubMed: 22698650) »

See 1 Publication for this product

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