概述

  • 产品名称
  • 描述
    小鼠单克隆抗体to GGT1
  • 经测试应用
    适用于: WB, IHC-P, ICC, ICC/IF, Flow Cytmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Recombinant fragment: TAHLSVVAED GSAVSATSTI NLYFGSKVRS PVSGILFNNE MDDFSSPSIT NEFGVPPSPA NFIQPGKQPL SSMCPTIMVG QDGQVRMVVG , corresponding to amino acids 381-471 of Human GGT1

性能

应用

Our Abpromise guarantee covers the use of ab55138 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 61 kDa.
IHC-P Use a concentration of 3 µg/ml.
ICC Use at an assay dependent concentration.
ICC/IF Use a concentration of 1 µg/ml.
Flow Cyt Use 1µg for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

靶标

  • 功能
    Initiates extracellular glutathione (GSH) breakdown, provides cells with a local cysteine supply and contributes to maintain intracelular GSH level. It is part of the cell antioxidant defense mechanism. Catalyzes the transfer of the glutamyl moiety of glutathione to amino acids and dipeptide acceptors. Alternatively, glutathione can be hydrolyzed to give Cys-Gly and gamma glutamate. Isoform 3 seems to be inactive.
  • 组织特异性
    Detected in fetal and adult kidney and liver, adult pancreas, stomach, intestine, placenta and lung. Isoform 3 is lung-specific. There are several other tissue-specific forms that arise from alternative promoter usage but that produce the same protein.
  • 通路
    Sulfur metabolism; glutathione metabolism.
  • 疾病相关
    Defects in GGT1 are a cause of glutathionuria (GLUTH) [MIM:231950]; also known as gamma-glutamyltranspeptidase deficiency. It is an autosomal recessive disease.
  • 序列相似性
    Belongs to the gamma-glutamyltransferase family.
  • 翻译后修饰
    N-glycosylated on both chains. Contains hexoses, hexosamines and sialic acid residues. Glycosylation profiles tested in kidney and liver tissues reveal the presence of tissue-specific and site-specific glycan composition, despite the overlap in composition among the N-glycans. A total of 36 glycan compositions, with 40 unique structures are observed. Up to 15 different glycans are observed at a single site, with site-specific variation in glycan composition. The difference in glycosylation profiles in the 2 tissues do not affect the enzyme activity.
  • 细胞定位
    Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CD224 antibody
    • D22S672 antibody
    • D22S732 antibody
    • Gamma glutamyl transpeptidase antibody
    • Gamma glutamyltransferase 1 antibody
    • Gamma glutamyltranspeptidase 1 antibody
    • Gamma-glutamyltransferase 1 antibody
    • Gamma-glutamyltranspeptidase 1 light chain antibody
    • GGT 1 antibody
    • GGT antibody
    • GGT1 antibody
    • GGT1_HUMAN antibody
    • Glutamyl transpeptidase antibody
    • Glutathione hydrolase 1 antibody
    • GTG antibody
    • Leukotriene C4 hydrolase antibody
    • MGC96892 antibody
    • MGC96904 antibody
    • MGC96963 antibody
    • OTTHUMP00000028921 antibody
    • OTTHUMP00000197959 antibody
    see all

图片

  • GGT1 antibody (ab55138) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human testis.


  • Predicted band size : 61 kDa
    GGT1 antibody (ab55138) at 1ug/lane + NIH/3T3 cell lysate at 25ug/lane.
  • ICC/IF image of ab55138 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab55138, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Overlay histogram showing HEK293 cells stained with ab55138 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab55138, 1µg/1x106 cells ) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HEK293 cells fixed with 100% methanol used under the same conditions.

    Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.

文献

This product has been referenced in:
  • Jiang Y  et al. GGsTOP increases migration of human periodontal ligament cells in vitro via reactive oxygen species pathway. Mol Med Rep 13:3813-20 (2016). IHC . Read more (PubMed: 27035100) »
  • Cruz-Orengo L  et al. Enhanced sphingosine-1-phosphate receptor 2 expression underlies female CNS autoimmunity susceptibility. J Clin Invest 124:2571-84 (2014). ICC/IF ; Mouse . Read more (PubMed: 24812668) »

See all 3 Publications for this product

客户评价及客户问答

Thank you for contacting us.

While we do not provide explicit expiration dates for our antibodies, we will guaranteethemfor six months from the date of purchase when stored according to our datasheet instructions. For ab41825 and ab55138, we ...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry
Sample
Mouse Cell (RAW 264.7)
Specification
RAW 264.7
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% Triton-X100 in 2% BSA for 15min
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 22°C
Username

Dr. Mahesh Shivananjappa

Verified customer

提交于 May 24 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Monocytes)
Specification
Monocytes
Fixative
BD Cytofix/Cytoperm 20 min
Permeabilization
Yes - BD Cytofix/Cytoperm 20 min
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
Username

Dr. Mahesh Shivananjappa

Verified customer

提交于 Feb 06 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Monocytes from Apheresis)
Loading amount
20 µg
Specification
Monocytes from Apheresis
Gel Running Conditions
Reduced Denaturing (8-16% Biorad Criterion Gel with 18 wells)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

提交于 Dec 12 2011

Thank you for contacting us with your question. Though the antibody has been tested for reactivity with human and mouse tissues, the protocol may need to be optimized for each tissue. I am not aware of anything specific for mouse staining, but I ma...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

注册