Customer reviews and FAQs

概述

  • 产品名称
    Anti-GGA2抗体
    参阅全部 GGA2 一抗
  • 描述
    兔多克隆抗体to GGA2
  • 特异性
    This antibody recognises two bands by Western at 75 and 37kD. This corresponds to results seen in Hela extracts in Dell'Angelica et al. 2000. The lower 37kD band is expected to correspond to the isoform described in NP_619581.
  • 经测试应用
    适用于: ICC/IF, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse
  • 免疫原

    Synthetic peptide:

    LSMYRRPGQAPPDQEAL

    conjugated to KLH, corresponding to amino acids 249-265 of Human GGA2.

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液
    Preservative: 0.01% Sodium Azide
    Constituents: 0.15M Sodium chloride, 0.02M Potassium phosphate. pH 7.2
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab10552 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use at an assay dependent dilution.
WB 1/500. Detects a band of approximately 75, 37 kDa (predicted molecular weight: 67 kDa).

靶标

  • 功能
    Plays a role in protein sorting and trafficking between the trans-Golgi network (TGN) and endosomes. Mediates the ARF-dependent recruitment of clathrin to the TGN and binds ubiquitinated proteins and membrane cargo molecules with a cytosolic acidic cluster-dileucine (AC-LL) motif.
  • 组织特异性
    Ubiquitously expressed.
  • 序列相似性
    Belongs to the GGA protein family.
    Contains 1 GAE domain.
    Contains 1 GAT domain.
    Contains 1 VHS domain.
  • 结构域
    The VHS domain functions as a recognition module for sorting signals composed of an acidic cluster followed by two leucines (AC-LL motif).
    The GAT domain is responsible for interaction with ARF-GTP, UBC and RABEP1. Required for recruitment to the TGN it prevents ARF-GTP hydrolysis.
    The unstructured hinge region contains clathrin-binding but no autoinhibitory (AC-LL) motifs.
    The GAE domain binds accessory proteins regulating GGAs function.
  • 翻译后修饰
    Ubiquitinated.
  • 细胞定位
    Golgi apparatus > trans-Golgi network membrane. Endosome membrane.
  • Information by UniProt
  • 数据库链接
    • 别名
      • 1200007E24Rik antibody
      • ADP ribosylation factor binding protein 2 antibody
      • ADP ribosylation factor binding protein GGA 2 antibody
      • ADP ribosylation factor binding protein GGA2 antibody
      • ADP-ribosylation factor-binding protein GGA2 antibody
      • ARF-binding protein 2 antibody
      • FLJ20966 antibody
      • Gamma adaptin related protein 2 antibody
      • gamma ear-containing antibody
      • Gamma-adaptin-related protein 2 antibody
      • GGA 2 antibody
      • Gga2 antibody
      • GGA2_HUMAN antibody
      • Golgi associated gamma adaptin ear containing ARF binding protein 2 antibody
      • Golgi localized gamma ear containing ARF binding protein 2 antibody
      • Golgi-localized antibody
      • KIAA1080 antibody
      • Vear antibody
      • VHS domain and ear domain containing protein antibody
      • VHS domain and ear domain of gamma adaptin antibody
      • VHS domain and ear domain of gamma-adaptin antibody
      see all

    Anti-GGA2 antibody 图像

    • Western blot - Anti-GGA2 antibody (ab10552)
      Western blot - Anti-GGA2 antibody (ab10552)


      Predicted band size : 67 kDa

      Western blot using ab10552 at 1/500. Lysates at 5µg per lane. Secondary antibody anti-rabbit HRP ab6721 at 1/5000.
           
      Lane 1: Hela nuclear
      Lane 2: Hela
      Lane 3: A431
      Lane 4: Jurkat
      Lane 5: 293

      Western blot using ab10552 at 1/500. Lysates at 5µg per lane. Secondary ab6721 at 1/5000. Lane 1: Hela nuclear Lane 2: Hela Lane 3: A431 Lane 4: Jurkat Lane 5: 293

    Anti-GGA2 antibody (ab10552)参考文献

    This product has been referenced in:
    • Herskowitz JH  et al. GGA1-mediated endocytic traffic of LR11/SorLA alters APP intracellular distribution and amyloid-ß production. Mol Biol Cell 23:2645-57 (2012). WB, ICC/IF ; Human . Read more (PubMed: 22621900) »
    • Lefrançois S & McCormick PJ The Arf GEF GBF1 is required for GGA recruitment to Golgi membranes. Traffic 8:1440-51 (2007). ICC/IF ; African green monkey . Read more (PubMed: 17666033) »

    See all 2 Publications for this product

    发表研究结果有使用 ab10552？请让我们知道，以便我们可以引用本数据表中的参考文章。

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    提交 ab10552 的 abreview 提交 ab10552 的问题

    1 Abreview or Q&A

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    Hi, I'm looking for a good GGA2 antibody for ICC in rabbit cultured cells or IHC in mouse tissue. I saw the reference on your page to a reasonably good ICC picture for Cos7 cells and thought it might be worthwhile to try in our cells- but was wondering...

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    Thank you for your enquiry. As far as we are aware, cross reactivity with mouse has not yet been tested for use with ab10552. All species cross-reactivity information is stated on Abcam product datasheets, and these are updated as soon as any new i...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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