Anti-GFP抗体[LGB-1] (Biotin) (ab42562)

概述

  • 产品名称
    Anti-GFP抗体[LGB-1] (Biotin)
    参阅全部 GFP 一抗
  • 描述
    小鼠单克隆抗体[LGB-1] to GFP (Biotin)
  • 偶联物
    Biotin
  • 特异性
    This antibody recognizes all forms of GFP from Aquorea victoria (i.e. GFP, EGFP, YFP and CFP).
  • 经测试应用
    适用于: WBmore details
  • 免疫原

    Recombinant full length protein corresponding to Escherichia coli GFP.
    Database link: P42212

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液
    Preservative: 0.1% Sodium Azide
    Constituents: 50% Glycerol, PBS
  • Concentration information loading...
  • 纯度
    Protein A purified
  • 克隆
    单克隆
  • 克隆编号
    LGB-1
  • 同种型
    IgG1
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab42562 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.5 µg/ml. Detects a band of approximately 27 kDa (predicted molecular weight: 27 kDa).

靶标

  • 相关性
    Function: Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca2+ -activated photoprotein aequorin.

    Subunit structure: Monomer.

    Tissue specificity: Photocytes.

    Post-translational modification: Contains a chromophore consisting of modified amino acid residues. The chromophore is formed by autocatalytic backbone condensation between Ser-65 and Gly-67, and oxidation of Tyr-66 to didehydrotyrosine. Maturation of the chromophore requires nothing other than molecular oxygen.

    Biotechnological use: Green fluorescent protein has been engineered to produce a vast number of variously colored mutants, fusion proteins, and biosensors. Fluorescent proteins and its mutated allelic forms, blue, cyan and yellow have become a useful and ubiquitous tool for making chimeric proteins, where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins. They have been expressed in most known cell types and are used as a noninvasive fluorescent marker in living cells and organisms. They enable a wide range of applications where they have functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions. Can also be used as a molecular thermometer, allowing accurate temperature measurements in fluids. The measurement process relies on the detection of the blinking of GFP using fluorescence correlation spectroscopy.

    Sequence similarities: Belongs to the GFP family.

    Biophysicochemical properties: Absorption: Abs(max)=395 nm
    Exhibits a smaller absorbance peak at 470 nm. The fluorescence emission spectrum peaks at 509 nm with a shoulder at 540 nm.
  • 别名
    • GFP antibody
    • Green fluorescent protein antibody

图片

  • All lanes : Anti-GFP antibody [LGB-1] (Biotin) (ab42562) at 0.5 µg/ml

    Lane 1 : 5ng GFP
    Lane 2 : 10ng GFP
    Lane 3 : 25ng GFP

    Secondary
    Neutravidin-HRP in TBST-1%BSA at 1/6000 dilution

    Predicted band size : 27 kDa
    Observed band size : 27 kDa

实验方案

文献

This product has been referenced in:
  • Brandt N  et al. B56{beta}, a regulatory subunit of protein phosphatase 2A, interacts with CALEB/NGC and inhibits CALEB/NGC-mediated dendritic branching. FASEB J : (2008). Read more (PubMed: 18385213) »

See 1 Publication for this product

客户评价及客户问答

I am sorry to confirm that they are all raised against full length protein, and the epitopes have regrettably not been mapped. We would therefore not be able to guarantee these will be detecting the C terminus of the GFP protein.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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