概述

  • 产品名称Anti-GFP抗体(Biotin)
    参阅全部 GFP 一抗
  • 描述
    兔多克隆抗体to GFP (Biotin)
  • 偶联物Biotin
  • 特异性This product is affinity purified antibody (ab6556) covalently linked to biotin. It is reactive against all variants of Aequorea victoria GFP such as S65T-GFP, RS-GFP, YFP, RFP and EGFP.
  • 经测试应用适用于: IHC-FoFr, WBmore details
  • 免疫原

    Highly purified recombinant full length GFP made in Escherichia coli

  • 常规说明This antibody is a biotin conjugated version of ab6556.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: None
    Constituents: 25mM Tris, 137mM Sodium chloride, pH 8
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明This antibody is an affinity purified rabbit anti-GFP antibody purified on an affinity chromatography column made with highly purified recombinant GFP.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab69313 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-FoFr Use at an assay dependent concentration. PubMed: 20824308
WB 1/25000. Detects a band of approximately 27 kDa (predicted molecular weight: 27 kDa). An unconjugated version of this antibody is available as ab6556. FITC conjugated version: ab66180. HRP conjugated version: ab69312. Sepharose version: ab69314. Magnetic beads version: ab69315.

靶标

  • 相关性Function: Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca2+ -activated photoprotein aequorin.

    Subunit structure: Monomer.

    Tissue specificity: Photocytes.

    Post-translational modification: Contains a chromophore consisting of modified amino acid residues. The chromophore is formed by autocatalytic backbone condensation between Ser-65 and Gly-67, and oxidation of Tyr-66 to didehydrotyrosine. Maturation of the chromophore requires nothing other than molecular oxygen.

    Biotechnological use: Green fluorescent protein has been engineered to produce a vast number of variously colored mutants, fusion proteins, and biosensors. Fluorescent proteins and its mutated allelic forms, blue, cyan and yellow have become a useful and ubiquitous tool for making chimeric proteins, where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins. They have been expressed in most known cell types and are used as a noninvasive fluorescent marker in living cells and organisms. They enable a wide range of applications where they have functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions. Can also be used as a molecular thermometer, allowing accurate temperature measurements in fluids. The measurement process relies on the detection of the blinking of GFP using fluorescence correlation spectroscopy.

    Sequence similarities: Belongs to the GFP family.

    Biophysicochemical properties: Absorption: Abs(max)=395 nm
    Exhibits a smaller absorbance peak at 470 nm. The fluorescence emission spectrum peaks at 509 nm with a shoulder at 540 nm.
  • 别名
    • GFP antibody
    • Green fluorescent protein antibody
    • yfp antibody

Anti-GFP antibody (Biotin) 图像



  • Predicted band size : 27 kDa
    Observed band size : 27 kDa

Anti-GFP antibody (Biotin) (ab69313)参考文献

This product has been referenced in:
  • Sztainberg Y  et al. An Anxiolytic Role for CRF Receptor Type 1 in the Globus Pallidus. J Neurosci 31:17416-24 (2011). Read more (PubMed: 22131403) »
  • Zhang H  et al. Multifunctional peptide-PEG intercalating conjugates: programmatic of gene delivery to the blood-brain barrier. Pharm Res 27:2528-43 (2010). IHC-FoFr ; Mouse . Read more (PubMed: 20824308) »

See all 2 Publications for this product

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