• 产品名称Anti-GFP抗体
    参阅全部 GFP 一抗
  • 描述
    鸡多克隆抗体to GFP
  • 特异性detects GFP
  • 经测试应用适用于: WB, IHC-Pmore details
  • 免疫原

    Recombinant full length GFP.


  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 50% Glycerol, PBS, pH 7.2
  • Concentration information loading...
  • 纯度Protein L purified
  • 克隆多克隆
  • 同种型IgY
  • 研究领域


Our Abpromise guarantee covers the use of ab101863 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/5000. Predicted molecular weight: 27 kDa.
IHC-P 1/500.


  • 相关性Function: Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca2+ -activated photoprotein aequorin.

    Subunit structure: Monomer.

    Tissue specificity: Photocytes.

    Post-translational modification: Contains a chromophore consisting of modified amino acid residues. The chromophore is formed by autocatalytic backbone condensation between Ser-65 and Gly-67, and oxidation of Tyr-66 to didehydrotyrosine. Maturation of the chromophore requires nothing other than molecular oxygen.

    Biotechnological use: Green fluorescent protein has been engineered to produce a vast number of variously colored mutants, fusion proteins, and biosensors. Fluorescent proteins and its mutated allelic forms, blue, cyan and yellow have become a useful and ubiquitous tool for making chimeric proteins, where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins. They have been expressed in most known cell types and are used as a noninvasive fluorescent marker in living cells and organisms. They enable a wide range of applications where they have functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions. Can also be used as a molecular thermometer, allowing accurate temperature measurements in fluids. The measurement process relies on the detection of the blinking of GFP using fluorescence correlation spectroscopy.

    Sequence similarities: Belongs to the GFP family.

    Biophysicochemical properties: Absorption: Abs(max)=395 nm
    Exhibits a smaller absorbance peak at 470 nm. The fluorescence emission spectrum peaks at 509 nm with a shoulder at 540 nm.
  • 别名
    • GFP antibody
    • Green fluorescent protein antibody
    • yfp antibody

Anti-GFP antibody 图像

  • Anti-GFP antibody (ab101863) at 1/5000 dilution + transgenic mice expressing the GFP gene product with IgY blocking reagent

    HRP-labeled goat anti-chicken antibodies

    Predicted band size : 27 kDa
  • ab101863, at a 1/500 dilution, staining GFP in transgenic mice expressing the GFP gene product by Immunohistochemistry.

Anti-GFP antibody (ab101863)参考文献

ab101863 has not yet been referenced specifically in any publications.

Product Wall

At Abcam, providing quality service is just as important as providing quality products. Lack of references does not mean the antibody has not been tested. Our Abpromise® ensures that you can trust our products to perform as stated on the datasheets, or...

Read More